Highlights on selected microscopy techniques to study zebrafish developmental biology

A Abu-Siniyeh, W Al-Zyoud - Laboratory Animal Research, 2020 - Springer
Bio-imaging is a tedious task when it concerns exploring cell functions, developmental
mechanisms, and other vital processes in vivo. Single-cell resolution is challenging due to …

Zebrafish vascular quantification: a tool for quantification of three-dimensional zebrafish cerebrovascular architecture by automated image analysis

EC Kugler, J Frost, V Silva, K Plant… - …, 2022 - journals.biologists.com
Zebrafish transgenic lines and light sheet fluorescence microscopy allow in-depth insights
into three-dimensional vascular development in vivo. However, quantification of the …

Vessel Metrics: A software tool for automated analysis of vascular structure in confocal imaging

SD McGarry, C Adjekukor, S Ahuja… - Microvascular …, 2024 - Elsevier
Images contain a wealth of information that is often under analyzed in biological studies.
Developmental models of vascular disease are a powerful way to quantify developmentally …

Application of the rolling ball algorithm to measure phase volume fraction from backscattered electron images

MCM Rodrigues, M Militzer - Materials Characterization, 2020 - Elsevier
The backscattered electron (BSE) mode in the scanning electron microscope has been
frequently used to characterize phases with different chemical compositions in metals and …

Enhancement and segmentation workflow for the developing zebrafish vasculature

E Kugler, K Plant, T Chico, P Armitage - Journal of Imaging, 2019 - mdpi.com
Zebrafish have become an established in vivo vertebrate model to study cardiovascular
development and disease. However, most published studies of the zebrafish vascular …

The effect of absent blood flow on the zebrafish cerebral and trunk vasculature

E Kugler, R Snodgrass, G Bowley, K Plant… - Vascular …, 2021 - vb.bioscientifica.com
The role of blood flow in vascular development is complex and context-dependent. In this
study, we quantify the effect of the lack of blood flow on embryonic vascular development on …

Segmentation of the zebrafish brain vasculature from light sheet fluorescence microscopy datasets

EC Kugler, A Rampun, TJA Chico, PA Armitage - bioRxiv, 2020 - biorxiv.org
Light sheet fluorescent microscopy allows imaging of zebrafish vascular development in
great detail. However, interpretation of data often relies on visual assessment and …

Validating segmentation of the zebrafish vasculature

E Kugler, T Chico, PA Armitage - … , MIUA 2019, Liverpool, UK, July 24–26 …, 2020 - Springer
The zebrafish is an established vertebrate model to study development and disease of the
cardiovascular system. Using transgenic lines and state-of-the-art microscopy it is possible …

Vessel Metrics: A python based software tool for automated analysis of vascular structure in confocal imaging

SD McGarry, C Adjekukor, S Ahuja, J Greysson-Wong… - bioRxiv, 2022 - biorxiv.org
Images contain a wealth of information that is often under analyzed in biological studies.
Developmental models of vascular disease are a powerful way to quantify developmentally …

Analytical Approaches for the Segmentation of the Zebrafish Brain Vasculature

EC Kugler, A Rampun, TJA Chico… - Current …, 2022 - Wiley Online Library
With advancements in imaging techniques, data visualization allows new insights into
fundamental biological processes of development and disease. However, although …