Designing materials to direct stem-cell fate

MP Lutolf, PM Gilbert, HM Blau - Nature, 2009 - nature.com
Proper tissue function and regeneration rely on robust spatial and temporal control of
biophysical and biochemical microenvironmental cues through mechanisms that remain …

[HTML][HTML] The extracellular matrix of hematopoietic stem cell niches

C Lee-Thedieck, P Schertl, G Klein - Advanced Drug Delivery Reviews, 2022 - Elsevier
Hematopoietic stem cells (HSCs) are the life-long source of all types of blood cells. Their
function is controlled by their direct microenvironment, the HSC niche in the bone marrow …

TrackMate 7: integrating state-of-the-art segmentation algorithms into tracking pipelines

D Ershov, MS Phan, JW Pylvänäinen, SU Rigaud… - Nature …, 2022 - nature.com
TrackMate is an automated tracking software used to analyze bioimages and is distributed
as a Fiji plugin. Here, we introduce a new version of TrackMate. TrackMate 7 is built to …

Maintenance of neural progenitor cell stemness in 3D hydrogels requires matrix remodelling

CM Madl, BL LeSavage, RE Dewi, CB Dinh… - Nature materials, 2017 - nature.com
Neural progenitor cell (NPC) culture within three-dimensional (3D) hydrogels is an attractive
strategy for expanding a therapeutically relevant number of stem cells. However, relatively …

Substrate elasticity regulates skeletal muscle stem cell self-renewal in culture

PM Gilbert, KL Havenstrite, KEG Magnusson, A Sacco… - Science, 2010 - science.org
Stem cells that naturally reside in adult tissues, such as muscle stem cells (MuSCs), exhibit
robust regenerative capacity in vivo that is rapidly lost in culture. Using a bioengineered …

Specification of haematopoietic stem cell fate via modulation of mitochondrial activity

N Vannini, M Girotra, O Naveiras, G Nikitin… - Nature …, 2016 - nature.com
Haematopoietic stem cells (HSCs) differ from their committed progeny by relying primarily on
anaerobic glycolysis rather than mitochondrial oxidative phosphorylation for energy …

Bringing TrackMate into the era of machine-learning and deep-learning

D Ershov, MS Phan, JW Pylvänäinen, SU Rigaud… - BioRxiv, 2021 - biorxiv.org
TrackMate is an automated tracking software used to analyze bioimages and distributed as
a Fiji plugin. Here we introduce a new version of TrackMate rewritten to improve …

In situ cell manipulation through enzymatic hydrogel photopatterning

KA Mosiewicz, L Kolb, AJ Van Der Vlies, MM Martino… - Nature materials, 2013 - nature.com
The physicochemical properties of hydrogels can be manipulated in both space and time
through the controlled application of a light beam. However, methods for hydrogel …

Artificial niche microarrays for probing single stem cell fate in high throughput

S Gobaa, S Hoehnel, M Roccio, A Negro, S Kobel… - Nature …, 2011 - nature.com
To understand the regulatory role of niches in maintaining stem-cell fate, multifactorial in
vitro models are required. These systems should enable analysis of biochemical and …

Prospective identification of hematopoietic lineage choice by deep learning

F Buggenthin, F Buettner, PS Hoppe, M Endele… - Nature …, 2017 - nature.com
Differentiation alters molecular properties of stem and progenitor cells, leading to changes in
their shape and movement characteristics. We present a deep neural network that …