This review analyzes a development in biochemistry, enzymology and biotechnology that
originally came as a surprise. Following the establishment of directed evolution of …

Enzymes have promising applications in chemicals, food, pharmaceuticals, and other variety
products because of their high efficiency, specificity, and environmentally friendly properties …

Pullulanases are important starch-debranching enzymes that mainly hydrolyze the α-1, 6-
glycosidic linkages in pullulan, starch, and oligosaccharides. Nevertheless, their practical …

β-Glycosidase from Sulfolobus solfataricus (SS-BGL) is a highly effective biocatalyst for the
synthesis of compound K (CK) from glycosylated protopanaxadiol ginsenosides. In order to …

Pullulanase is a starch-debranching enzyme that hydrolyzes side chain of starch,
oligosaccharides and pullulan. Nevertheless, the limited activities of pullulanases constrain …

Wine lees is a low value biomass resource rich in cellulose, with great potential for
producing organic fertilizers and chemicals. However, the high acidity of wine lees limits the …

Vanillin dehydrogenase (VDH) has recently come forward as an important enzyme for the
commercial production of vanillic acid from vanillin in a one-step enzymatic process …

Lipases catalyze the synthesis of biodiesel, which is an important renewable alternative
energy source. Cost-efficient conversion of waste acidified oil to biodiesel entails acid …

Serving as a vital medical intermediate and an environmentally-friendly preservative, D-PLA
exhibits substantial potential across various industries. In this report, the urgent need for …

Glucose isomerase is generally used in the industrial production of high-fructose corn syrup,
and a heat-and acid-resistant glucose isomerase is preferred. However, most glucose …