Protein production using the baculovirus‐insect cell expression system

A Contreras‐Gómez, A Sánchez‐Mirón… - Biotechnology …, 2014 - Wiley Online Library
The baculovirus‐insect cell expression system is widely used in producing recombinant
proteins. This review is focused on the use of this expression system in developing …

Toward system-level understanding of baculovirus–host cell interactions: from molecular fundamental studies to large-scale proteomics approaches

F Monteiro, N Carinhas, MJT Carrondo… - Frontiers in …, 2012 - frontiersin.org
Baculoviruses are insect viruses extensively exploited as eukaryotic protein expression
vectors. Molecular biology studies have provided exciting discoveries on virus–host …

Biodegradation of triphenylmethane dye crystal violet by Cedecea davisae

DJ Cao, JJ Wang, Q Zhang, YZ Wen, B Dong… - … Acta Part A: Molecular …, 2019 - Elsevier
The present study focuses on the biodegradation of triphenylmethane dye crystal violet (CV)
by Cedecea davisae. The degradation of CV was evaluated via ultraviolet absorbance at …

[HTML][HTML] The effect of different insect cell culture media on the efficiency of protein production by Spodoptera frugiperda cells

L Käßer, J Harnischfeger, D Salzig… - Electronic Journal of …, 2022 - Elsevier
Abstract Background Spodoptera frugiperda (Sf-9) cells are often used to produce
recombinant proteins by transient expression. The culture medium contributes to the …

Dynamics of the interaction between cotton bollworm Helicoverpa armigera and nucleopolyhedrovirus as revealed by integrated transcriptomic and proteomic …

L Xing, C Yuan, M Wang, Z Lin, B Shen, Z Hu… - Molecular & Cellular …, 2017 - ASBMB
Over the past decades, Helicoverpa armigera nucleopolyhedrovirus (HearNPV) has been
widely used for biocontrol of cotton bollworm, which is one of the most destructive pest …

Combining stable insect cell lines with baculovirus-mediated expression for multi-HA influenza VLP production

DP Sequeira, R Correia, MJT Carrondo, A Roldão… - Vaccine, 2018 - Elsevier
Safer and broadly protective vaccines are needed to cope with the continuous evolution of
circulating influenza virus strains and promising approaches based on the expression of …

Genome scale transcriptomics of baculovirus-insect interactions

Q Nguyen, LK Nielsen, S Reid - Viruses, 2013 - mdpi.com
Baculovirus-insect cell technologies are applied in the production of complex proteins,
veterinary and human vaccines, gene delivery vectors ‚and biopesticides. Better …

Transcriptome analysis of Sf9 insect cells during production of recombinant Adeno‐associated virus

N Virgolini, R Hagan, R Correia, M Silvano… - Biotechnology …, 2023 - Wiley Online Library
The insect cell‐baculovirus expression vector system (IC‐BEVS) has emerged as an
alternative time‐and cost‐efficient production platform for recombinant Adeno‐associated …

Systems biotechnology of animal cells: the road to prediction

N Carinhas, R Oliveira, PM Alves, MJT Carrondo… - Trends in …, 2012 - cell.com
A central concern of biopharmaceutical R&D is the production of sufficient quantities of
recombinant products from manufacturing processes based on animal cell culture. The way …

Impact of dual‐baculovirus infection on the Sf9 insect cell transcriptome during rAAV production using single‐cell RNA‐seq

N Virgolini, M Silvano, R Hagan… - Biotechnology and …, 2023 - Wiley Online Library
The insect cell‐baculovirus expression vector system (IC‐BEVS) has shown to be a powerful
platform to produce complex biopharmaceutical products, such as recombinant proteins and …