We developed a highly sensitive and specific LAMP assay for Escherichia coli. It does not
require DNA extraction and can detect as few as 10 copies. It detected all 36 of 36 E. coli …

Influence of surveillance renal allograft biopsy on diagnosis and prognosis of polyomavirus-
associated nephropathy. Background Polyomavirus-associated nephropathy (PVAN) is an …

We have compared various sample preparation methods for the PCR diagnosis of visceral
leishmaniasis (VL) using peripheral blood samples and tested the influence of these …

Visceral leishmaniasis is a serious and debilitating infection with high fatality rate in tropical
and subtropical countries. As clinical symptoms of visceral leishmaniasis are not so specific …

PCR's simplicity as a molecular technique is, in some ways, responsible for the huge
amount of innovation that surrounds it, as researchers continually think of new ways to …

Background: The majority of the human population is infected with two human
polyomaviruses BK virus (BKV) and JC virus (JCV) during childhood. After initial infection …

The sensitivity and reliability of PCR for diagnostic and research purposes require efficient
unbiased procedures of extraction and purification of nucleic acids. One of the major …

4.483, 920 A 1 1/1984 Gillespie et al. 4,539,256 A 9/1985 Shipman 4,565,663 A 1, 1986
Errede et al. 4,619,897 A 10, 1986 Hato et al. 4,726,989 A 2f1988 Mrozinski 4,737,560 A 4 …

Identification of Acanthamoeba cysts and trophozoites in ocular tissues requires
considerable expertise and is often time-consuming. An 18S rRNA gene-based PCR test …

A technique used to amplify the number of copies of a specific region of DNA, the
polymerase chain reaction (PCR) is at the forefront of the dramatic development of …