Single‐cell analysis using droplet microfluidics
Droplet microfluidics has revolutionized the study of single cells. The ability to
compartmentalize cells within picoliter droplets in microfluidic devices has opened up a wide …
compartmentalize cells within picoliter droplets in microfluidic devices has opened up a wide …
MYC: connecting selective transcriptional control to global RNA production
TR Kress, A Sabò, B Amati - Nature Reviews Cancer, 2015 - nature.com
Two opposing models have been proposed to describe the function of the MYC oncoprotein
in shaping cellular transcriptomes: one posits that MYC amplifies transcription at all active …
in shaping cellular transcriptomes: one posits that MYC amplifies transcription at all active …
Metabolic regulation of species-specific developmental rates
M Diaz-Cuadros, TP Miettinen, OS Skinner, D Sheedy… - Nature, 2023 - nature.com
Animals display substantial inter-species variation in the rate of embryonic development
despite a broad conservation of the overall sequence of developmental events. Differences …
despite a broad conservation of the overall sequence of developmental events. Differences …
Polyploidy, the nucleotype, and novelty: the impact of genome doubling on the biology of the cell
Polyploidy is a key mechanism of genome evolution and speciation, particularly in plants.
Many aspects of polyploidy have been elucidated with the tools that have become available …
Many aspects of polyploidy have been elucidated with the tools that have become available …
[HTML][HTML] Single mammalian cells compensate for differences in cellular volume and DNA copy number through independent global transcriptional mechanisms
O Padovan-Merhar, GP Nair, AG Biaesch, A Mayer… - Molecular cell, 2015 - cell.com
Individual mammalian cells exhibit large variability in cellular volume, even with the same
absolute DNA content, and so must compensate for differences in DNA concentration in …
absolute DNA content, and so must compensate for differences in DNA concentration in …
Selective transcriptional regulation by Myc in cellular growth control and lymphomagenesis
A Sabo, TR Kress, M Pelizzola, S De Pretis, MM Gorski… - Nature, 2014 - nature.com
The c-myc proto-oncogene product, Myc, is a transcription factor that binds thousands of
genomic loci. Recent work suggested that rather than up-and downregulating selected …
genomic loci. Recent work suggested that rather than up-and downregulating selected …
[HTML][HTML] Mitochondrial potentiation ameliorates age-related heterogeneity in hematopoietic stem cell function
E Mansell, V Sigurdsson, E Deltcheva, J Brown… - Cell Stem Cell, 2021 - cell.com
Aging is associated with reduced fitness and increased myeloid bias of the hematopoietic
stem cell (HSC) compartment, causing increased risk of immune compromise, anemia, and …
stem cell (HSC) compartment, causing increased risk of immune compromise, anemia, and …
[HTML][HTML] Control of transcript variability in single mammalian cells
N Battich, T Stoeger, L Pelkmans - Cell, 2015 - cell.com
A central question in biology is whether variability between genetically identical cells
exposed to the same culture conditions is largely stochastic or deterministic. Using image …
exposed to the same culture conditions is largely stochastic or deterministic. Using image …
[HTML][HTML] Single-cell entropy for accurate estimation of differentiation potency from a cell's transcriptome
AE Teschendorff, T Enver - Nature communications, 2017 - nature.com
The ability to quantify differentiation potential of single cells is a task of critical importance.
Here we demonstrate, using over 7,000 single-cell RNA-Seq profiles, that differentiation …
Here we demonstrate, using over 7,000 single-cell RNA-Seq profiles, that differentiation …
[HTML][HTML] Bet hedging in yeast by heterogeneous, age-correlated expression of a stress protectant
Genetically identical cells grown in the same culture display striking cell-to-cell
heterogeneity in gene expression and other traits. A crucial challenge is to understand how …
heterogeneity in gene expression and other traits. A crucial challenge is to understand how …