Roughly one quarter of all genes code for integral membrane proteins that are inserted into
the plasma membrane of prokaryotes or the endoplasmic reticulum membrane of …

Advances over the past 25 years have revealed much about how the structural properties of
membranes and associated proteins are linked to the thermodynamics and kinetics of …

Elucidating the wiring diagram of the human cell is a central goal of the postgenomic era.
We combined genome engineering, confocal live-cell imaging, mass spectrometry, and data …

Most membrane proteins are synthesized on endoplasmic reticulum (ER)-bound ribosomes
docked at the translocon, a heterogeneous ensemble of transmembrane factors operating …

Most eukaryotic multipass membrane proteins are inserted into the membrane of the
endoplasmic reticulum. Their transmembrane domains (TMDs) are thought to be inserted co …

Mammals encode∼ 5,000 integral membrane proteins that need to be inserted in a defined
topology at the endoplasmic reticulum (ER) membrane by mechanisms that are incompletely …

Membrane proteins with multiple transmembrane domains play critical roles in cell
physiology, but little is known about the machinery coordinating their biogenesis at the …

A defining step in the biogenesis of a membrane protein is the insertion of its hydrophobic
transmembrane helices into the lipid bilayer. The nine-subunit endoplasmic reticulum (ER) …

The endoplasmic reticulum (ER) membrane complex (EMC) cooperates with the Sec61
translocon to co-translationally insert a transmembrane helix (TMH) of many multi-pass …

A central question in the post-genomic era is how genes interact to form biological
pathways. Measurements of gene dependency across hundreds of cell lines have been …