Integration of Agrobacterium T-DNA into the Plant Genome

SB Gelvin - Annual review of genetics, 2017 - annualreviews.org
Agrobacterium strains transfer a single-strand form of T-DNA (T-strands) and Virulence (Vir)
effector proteins to plant cells. Following transfer, T-strands likely form complexes with Vir …

New insights into an old story: Agrobacterium‐induced tumour formation in plants by plant transformation

A Pitzschke, H Hirt - The EMBO Journal, 2010 - embopress.org
Agrobacterium tumefaciens causes tumour formation in plants. Plant signals induce in the
bacteria the expression of a range of virulence (Vir) proteins and the formation of a type IV …

INFLORESCENCE DEFICIENT IN ABSCISSION Controls Floral Organ Abscission in Arabidopsis and Identifies a Novel Family of Putative Ligands in Plants

MA Butenko, SE Patterson, PE Grini, GE Stenvik… - The Plant …, 2003 - academic.oup.com
Abscission is an active process that enables plants to shed unwanted organs. Because the
purpose of the flower is to facilitate pollination, it often is abscised after fertilization. We have …

Approaches to minimize variation of transgene expression in plants

KMJ Butaye, BPA Cammue, SL Delauré… - Molecular …, 2005 - Springer
Genetic transformation of plants has become a widely used technology that serves multiple
purposes in plant biology research. However, considerable variation of transgene …

Rapid Metabolism of Glucose Detected with FRET Glucose Nanosensors in Epidermal Cells and Intact Roots of Arabidopsis RNA-Silencing Mutants

K Deuschle, B Chaudhuri, S Okumoto, I Lager… - The Plant …, 2006 - academic.oup.com
Genetically encoded glucose nanosensors have been used to measure steady state
glucose levels in mammalian cytosol, nuclei, and endoplasmic reticulum. Unfortunately, the …

Stable high‐level transgene expression in Arabidopsis thaliana using gene silencing mutants and matrix attachment regions

KMJ Butaye, IJWM Goderis, PFJ Wouters… - The plant …, 2004 - Wiley Online Library
Basic and applied research involving transgenic plants often requires consistent high‐level
expression of transgenes. However, high inter‐transformant variability of transgene …

[PDF][PDF] Transformation-induced mutations in transgenic plants: analysis and biosafety implications

AK Wilson, JR Latham… - Biotechnology and genetic …, 2006 - Taylor & Francis
Plant trans Ibrmat ion has become an essential tool for plant molecular biologists and.
almost simultaneously. iransgenic plants have become a major focus of many plant …

How effective is T-DNA insertional mutagenesis in Arabidopsis?

YH Wang - Journal of Biochemical Technology, 2008 - jbiochemtech.com
To evaluate the effectiveness of T-DNA insertion in knocking out a gene, 1084 published
Arabidopsis thaliana insertion mutants representing 755 genes in 648 publications were …

T-DNA integration in Arabidopsis chromosomes. Presence and origin of filler DNA sequences

P Windels, S De Buck, E Van Bockstaele… - Plant …, 2003 - academic.oup.com
To investigate the relationship between T-DNA integration and double-stranded break
(DSB) repair in Arabidopsis, we studied 67 T-DNA/plant DNA junctions and 13 T-DNA/T …

Enhanced single copy integration events in corn via particle bombardment using low quantities of DNA

BA Lowe, N Shiva Prakash, M Way, MT Mann… - Transgenic …, 2009 - Springer
Transgene copy number is an important criterion for determining the utility of transgenic
events. Single copy integration events are highly desirable when the objective is to produce …