Escherichia coli offers a means for the rapid and economical production of recombinant
proteins. These advantages, coupled with a wealth of biochemical and genetic knowledge …

A common limitation of recombinant protein production in bacteria is the formation of
insoluble protein aggregates known as inclusion bodies. The propensity of a given protein to …

Background In the search for generic expression strategies for mammalian protein families
several bacterial expression vectors were examined for their ability to promote high yields of …

The efficient in vivo folding of many heterologous proteins is a major bottleneck of high level
production in bacterial hosts and simple optimisation protocols have not been available yet …

Pre-mRNA splicing is executed by the spliceosome, which has eight major functional states
each with distinct composition. Five of these eight human spliceosomal complexes, all …

Human granulocyte‐colony stimulating factor (hG‐CSF), an important biopharmaceutical
drug used in oncology, is currently produced mainly in Escherichia coli. Expression of …

Understanding the genetic basis of adaptation is a central problem in biology. However,
revealing the underlying molecular mechanisms has been challenging as changes in fitness …

Insufficient availability of molecular chaperones is observed as a major bottleneck for proper
protein folding in recombinant protein production. Therefore, co-production of selected sets …

Bacterial degradation of the sugar sulfoquinovose (SQ, 6-deoxy-6-sulfoglucose) produced
by plants, algae, and cyanobacteria, is an important component of the biogeochemical …

Publisher Summary In 1975 Prouty et al. first described the formation of dense, amorphous
intracellular granules in Escherichia coli cells grown in the presence of the amino acid …