Advances in Escherichia coli production of therapeutic proteins

JR Swartz - Current opinion in biotechnology, 2001 - Elsevier
Escherichia coli offers a means for the rapid and economical production of recombinant
proteins. These advantages, coupled with a wealth of biochemical and genetic knowledge …

Protein quality in bacterial inclusion bodies

S Ventura, A Villaverde - TRENDS in Biotechnology, 2006 - cell.com
A common limitation of recombinant protein production in bacteria is the formation of
insoluble protein aggregates known as inclusion bodies. The propensity of a given protein to …

Production of soluble mammalian proteins in Escherichia coli: identification of protein features that correlate with successful expression

MR Dyson, SP Shadbolt, KJ Vincent, RL Perera… - BMC …, 2004 - Springer
Background In the search for generic expression strategies for mammalian protein families
several bacterial expression vectors were examined for their ability to promote high yields of …

Inclusion bodies: formation and utilisation

B Fahnert, H Lilie, P Neubauer - Physiological Stress Responses in …, 2004 - Springer
The efficient in vivo folding of many heterologous proteins is a major bottleneck of high level
production in bacterial hosts and simple optimisation protocols have not been available yet …

Structures of the human spliceosomes before and after release of the ligated exon

X Zhang, X Zhan, C Yan, W Zhang, D Liu, J Lei, Y Shi - Cell research, 2019 - nature.com
Pre-mRNA splicing is executed by the spliceosome, which has eight major functional states
each with distinct composition. Five of these eight human spliceosomal complexes, all …

Production of nonclassical inclusion bodies from which correctly folded protein can be extracted

S Jevševar, V Gaberc‐Porekar, I Fonda… - Biotechnology …, 2005 - Wiley Online Library
Human granulocyte‐colony stimulating factor (hG‐CSF), an important biopharmaceutical
drug used in oncology, is currently produced mainly in Escherichia coli. Expression of …

Regulatory and metabolic rewiring during laboratory evolution of ethanol tolerance in E. coli

H Goodarzi, BD Bennett, S Amini… - Molecular systems …, 2010 - embopress.org
Understanding the genetic basis of adaptation is a central problem in biology. However,
revealing the underlying molecular mechanisms has been challenging as changes in fitness …

Side effects of chaperone gene co-expression in recombinant protein production

M Martínez-Alonso, E García-Fruitós… - Microbial cell …, 2010 - Springer
Insufficient availability of molecular chaperones is observed as a major bottleneck for proper
protein folding in recombinant protein production. Therefore, co-production of selected sets …

Environmental and intestinal phylum Firmicutes bacteria metabolize the plant sugar sulfoquinovose via a 6-deoxy-6-sulfofructose transaldolase pathway

B Frommeyer, AW Fiedler, SR Oehler, BT Hanson… - Iscience, 2020 - cell.com
Bacterial degradation of the sugar sulfoquinovose (SQ, 6-deoxy-6-sulfoglucose) produced
by plants, algae, and cyanobacteria, is an important component of the biogeochemical …

[3] Isolating inclusion bodies from bacteria

G Georgiou, P Valax - Methods in enzymology, 1999 - Elsevier
Publisher Summary In 1975 Prouty et al. first described the formation of dense, amorphous
intracellular granules in Escherichia coli cells grown in the presence of the amino acid …