Real-time PCR is the method of choice in many laboratories for diagnostic and food
applications. This technology merges the polymerase chain reaction chemistry with the use …

Liquid biopsy based on cell-free DNA (cfDNA) enables non-invasive dynamic assessment of
disease status in patients with cancer, both in the early and advanced settings. The analysis …

Enhancers control the correct temporal and cell-type-specific activation of gene expression
in multicellular eukaryotes. Knowing their properties, regulatory activity and targets is crucial …

CrAssphages represent the most abundant virus in the human gut microbiota, but the lack of
available genome sequences for comparison has kept them enigmatic. Recently, sequence …

Optimization of primer sequences for polymerase chain reaction (PCR) and quantitative
PCR (qPCR) and reaction conditions remains an experimental challenge. We have …

Long noncoding RNAs (lncRNAs) represent a big category of noncoding RNA molecules,
and increasing studies have shown that they play important roles in various critical …

ABSTRACT PrimerBank (http://pga. mgh. harvard. edu/primerbank/) is a public resource for
the retrieval of human and mouse primer pairs for gene expression analysis by PCR and …

Aim The ulcerative phase of oral mucositis following radiotherapy/chemotherapy for oral
cancer colonizes bacteria, fungi and viruses. The role of a microbiota, specifically bacterial …

Microplastic particles are ubiquitous not only in marine but also in freshwater ecosystems.
However, the impacts of microplastics, consisting of a large variety of synthetic polymers, on …

Satellite DNA (satDNA) is a major component yet the great unknown of eukaryote genomes
and clearly underrepresented in genome sequencing projects. Here we show the high …