MiFish metabarcoding: a high-throughput approach for simultaneous detection of multiple fish species from environmental DNA and other samples

M Miya, RO Gotoh, T Sado - Fisheries Science, 2020 - Springer
We reviewed the current methodology and practices of the DNA metabarcoding approach
using a universal PCR primer pair MiFish, which co-amplifies a short fragment of fish DNA …

Fishing for fish environmental DNA: Ecological applications, methodological considerations, surveying designs, and ways forward

M Yao, S Zhang, Q Lu, X Chen, SY Zhang… - Molecular …, 2022 - Wiley Online Library
Vast global declines of freshwater and marine fish diversity and population abundance pose
serious threats to both ecosystem sustainability and human livelihoods. Environmental DNA …

Environmental DNA shedding and decay rates from diverse animal forms and thermal regimes

E Andruszkiewicz Allan, WG Zhang… - Environmental …, 2021 - Wiley Online Library
Environmental DNA (eDNA) analysis from water samples is a promising new method to
identify both targeted species and whole communities of aquatic organisms. However, the …

[HTML][HTML] eDNA in subterranean ecosystems: Applications, technical aspects, and future prospects

M Saccò, MT Guzik, M van der Heyde, P Nevill… - Science of the Total …, 2022 - Elsevier
Monitoring of biota is pivotal for the assessment and conservation of ecosystems.
Environments worldwide are being continuously and increasingly exposed to multiple …

Environmental RNA as a tool for marine community biodiversity assessments

MS Giroux, JR Reichman, T Langknecht… - Scientific Reports, 2022 - nature.com
Microscopic organisms are often overlooked in traditional diversity assessments due to the
difficulty of identifying them based on morphology. Metabarcoding is a method for rapidly …

Systematic review and meta‐analysis: Water type and temperature affect environmental DNA decay

PD Lamb, VG Fonseca, DL Maxwell… - Molecular Ecology …, 2022 - Wiley Online Library
Environmental DNA (eDNA) has been used in a variety of ecological studies and
management applications. The rate at which eDNA decays has been widely studied but at …

Complex interactions between environmental DNA (eDNA) state and water chemistries on eDNA persistence suggested by meta‐analyses

T Jo, T Minamoto - Molecular Ecology Resources, 2021 - Wiley Online Library
Understanding the processes of environmental DNA (eDNA) persistence and degradation is
essential to determine the spatiotemporal scale of eDNA signals and accurately estimate …

Linking the state of environmental DNA to its application for biomonitoring and stock assessment: Targeting mitochondrial/nuclear genes, and different DNA fragment …

T Jo, K Takao, T Minamoto - Environmental DNA, 2022 - Wiley Online Library
Environmental DNA (eDNA) analysis is a revolutionary tool for non‐invasive, cost‐effective,
and highly sensitive monitoring of species distribution and abundance; however, there …

Environmental conditions influence eDNA particle size distribution in aquatic systems

MA Barnes, WL Chadderton, CL Jerde… - Environmental …, 2021 - Wiley Online Library
Abstract Knowledge about the size of environmental DNA (eDNA) and eDNA‐bearing
particles in aquatic environments is integral to efficient and sensitive analyses. To explore …

Are environmental DNA methods ready for aquatic invasive species management?

AJ Sepulveda, NM Nelson, CL Jerde… - Trends in ecology & …, 2020 - cell.com
Multiple studies have demonstrated environmental (e) DNA detections of rare and invasive
species. However, invasive species managers struggle with using eDNA results because …