Transmission Kikuchi diffraction (TKD), also known as transmission electron backscatter
diffraction (t-EBSD), has received significant interest for the characterisation of …

Cryo-electron tomography (CET) is ideally suited for bridging the resolution gap between
molecular and cellular structural studies. However, CET is limited to a sample thickness …

The bacterial division apparatus catalyses the synthesis and remodelling of septal
peptidoglycan (sPG) to build the cell wall layer that fortifies the daughter cell poles …

Chloroplast function is orchestrated by the organelle's intricate architecture. By combining
cryo-focused ion beam milling of vitreous Chlamydomonas cells with cryo-electron …

Recent advances have made cryogenic (cryo) electron microscopy a key technique to
achieve near-atomic-resolution structures of biochemically isolated macromolecular …

The ability to precisely control the preparation of biological samples for investigations by
electron cryo-microscopy is becoming increasingly important for ultrastructural imaging in …

Since the end of the last millennium, the focused ion beam scanning electron microscopy
(FIB‐SEM) has progressively found use in biological research. This instrument is a scanning …

The introduction of cryo‐techniques to the focused ion‐beam scanning electron microscope
(FIB‐SEM) has brought new opportunities to study frozen, hydrated samples from the field of …

Electron cryotomography is able to visualize macromolecular complexes in their cellular
context, in a frozen-hydrated state, and in three dimensions. The method, however, is limited …

The abilities of scanning electron microscopy (SEM) and focused ion beam (FIB) milling for
obtaining high‐resolution images from top surfaces, cross‐sectional surfaces, and even in …