Oriented immobilization of biologically active proteins as a tool for revealing protein interactions and function

J Turkova - Journal of Chromatography B: Biomedical Sciences …, 1999 - Elsevier
The advantages of oriented immobilization of biologically active proteins are good steric
accessibilities of active binding sites and increased stability. This not only may help to …

Immobilized microfluidic enzymatic reactors

J Křenková, F Foret - Electrophoresis, 2004 - Wiley Online Library
The use of enzymes for cleavage, synthesis or chemical modification represents one of the
most common processes used in biochemical and molecular biology laboratories. The …

Capillary electrophoresis

CA Monnig, RT Kennedy - Analytical chemistry, 1994 - ACS Publications
This is the third fundamental review of capillary electro-phoresis (CE) and micellar
electrokinetic chromatography (MEKC). The focus of this review will be the work which has …

Microsystem for rapid DNA sequencing

SA Soper, JD Davies, Y Vladimirsky - US Patent 5,846,727, 1998 - Google Patents
[57] ABSTRACT A system is disclosed for the rapid and cost-effective sequencing of DNA.
There are three principal components of the system:(1) a microreactor, Which prepares DNA …

Enzymatic microreactors in chemical analysis and kinetic studies

PL Urban, DM Goodall, NC Bruce - Biotechnology Advances, 2006 - Elsevier
The fields of application of microreactors are becoming wider every year. A considerable
number of papers have been published recently reporting successful application of …

Organic− inorganic hybrid silica monolith based immobilized trypsin reactor with high enzymatic activity

J Ma, Z Liang, X Qiao, Q Deng, D Tao, L Zhang… - Analytical …, 2008 - ACS Publications
A novel kind of immobilized trypsin reactor based on organic− inorganic hybrid silica
monoliths has been developed. With the presence of cetyltrimethyl ammonium bromide …

Affinity capillary electrophoresis: important application areas and some recent developments

NHH Heegaard, S Nilsson, NA Guzman - Journal of Chromatography B …, 1998 - Elsevier
Affinity capillary electrophoresis (ACE) is a broad term referring to the separation by capillary
electrophoresis of substances that participate in specific or non-specific affinity interactions …

Immobilized trypsin systems coupled on‐line to separation methods: Recent developments and analytical applications

G Massolini, E Calleri - Journal of separation science, 2005 - Wiley Online Library
The ability to rapidly and efficiently digest and identify an unknown protein is of great utility
for proteome studies. Identification of proteins via peptide mapping is generally …

Peptide mapping of proteins by capillary electromigration methods

V Kašička - Journal of Separation Science, 2022 - Wiley Online Library
This review article provides a wide overview of important developments and applications of
capillary electromigration methods in the area of peptide mapping of proteins in the period …

Immobilized enzyme reactors in proteomics

J Ma, L Zhang, Z Liang, Y Shan, Y Zhang - TrAC Trends in Analytical …, 2011 - Elsevier
Fast, efficient characterization of proteins is becoming one of the hottest topics in the
bioanalytical community, especially for large-scale proteomic studies. As an attractive …