Role of the extremolytes ectoine and hydroxyectoine as stress protectants and nutrients: genetics, phylogenomics, biochemistry, and structural analysis

L Czech, L Hermann, N Stöveken, AA Richter… - Genes, 2018 - mdpi.com
Fluctuations in environmental osmolarity are ubiquitous stress factors in many natural
habitats of microorganisms, as they inevitably trigger osmotically instigated fluxes of water …

Thermophilic methanotrophs: In hot pursuit

KM Houghton, CR Carere, MB Stott… - FEMS Microbiology …, 2019 - academic.oup.com
Methane is a potent greenhouse gas responsible for 20–30% of global climate change
effects. The global methane budget is∼ 500–600 Tg y− 1, with the majority of methane …

Strangers in the archaeal world: osmostress‐responsive biosynthesis of ectoine and hydroxyectoine by the marine thaumarchaeon Nitrosopumilus maritimus

N Widderich, L Czech, FJ Elling… - Environmental …, 2016 - Wiley Online Library
Ectoine and hydroxyectoine are compatible solutes widely synthesized by members of the
Bacteria to cope with high osmolarity surroundings. Inspection of 557 archaeal genomes …

Ectoine and 5-hydroxyectoine accumulation in the halophile Virgibacillus halodenitrificans PDB-F2 in response to salt stress

P Tao, H Li, Y Yu, J Gu, Y Liu - Applied microbiology and biotechnology, 2016 - Springer
The moderately halophilic bacterium Virgibacillus halodenitrificans PDB-F2 copes with
salinity by synthesizing or taking up compatible solutes. The main compatible solutes in this …

Understanding the interplay of carbon and nitrogen supply for ectoines production and metabolic overflow in high density cultures of Chromohalobacter salexigens

MJ Salar-García, V Bernal, JM Pastor, M Salvador… - Microbial cell …, 2017 - Springer
Abstract Background The halophilic bacterium Chromohalobacter salexigens has been
proposed as promising cell factory for the production of the compatible solutes ectoine and …

Quantitative RNA-seq Analysis Unveils Osmotic and Thermal Adaptation Mechanisms Relevant for Ectoine Production in Chromohalobacter salexigens

M Salvador, M Argandoña, E Naranjo… - Frontiers in …, 2018 - frontiersin.org
Quantitative RNA sequencing (RNA-seq) and the complementary phenotypic assays were
implemented to investigate the transcriptional responses of Chromohalobacter salexigens to …

Halomonas sedimenti sp. nov., a Halotolerant Bacterium Isolated from Deep-Sea Sediment of the Southwest Indian Ocean

X Qiu, L Yu, X Cao, H Wu, G Xu, X Tang - Current Microbiology, 2021 - Springer
A Gram-staining-negative, aerobic, flagellated, motile, rod-shaped, halophilic bacterium QX-
2 T was isolated from the deep-sea sediment of the Southwest Indian Ocean at a depth of …

New insights into hydroxyectoine synthesis and its transcriptional regulation in the broad‐salt growing halophilic bacterium Chromohalobacter salexigens

M Argandona, F Piubeli, M Reina‐Bueno… - Microbial …, 2021 - Wiley Online Library
Elucidating the mechanisms controlling the synthesis of hydroxyectoine is important to
design novel genetic engineering strategies for optimizing the production of this …

Promoter engineering for high ectoine production in a lower saline medium by Halomonas hydrothermalis Y2

S Li, Y Shang, Q Zhao, Y Liu, X Dong, W Wang… - Biotechnology …, 2021 - Springer
Objectives For the stress from fermenters, downstream processing equipment, and
wastewater treatment to be alleviated, lowering salt-dependence in the ectoine synthesis …

[HTML][HTML] Engineering the Salt-Inducible Ectoine Promoter Region of Halomonas elongata for Protein Expression in a Unique Stabilizing Environment

LM Stiller, EA Galinski, EMHJ Witt - Genes, 2018 - mdpi.com
It has been firmly established that organic osmolytes (compatible solutes) of halophilic
Bacteria and Archaea have positive effects on conformation and activity of proteins, and may …