Ultrasequencing of the meiofaunal biosphere: practice, pitfalls and promises

S Creer, VG Fonseca, DL Porazinska… - Molecular …, 2010 - Wiley Online Library
Biodiversity assessment is the key to understanding the relationship between biodiversity
and ecosystem functioning, but there is a well‐acknowledged biodiversity identification gap …

[HTML][HTML] Multi-template polymerase chain reaction

E Kalle, M Kubista, C Rensing - Biomolecular Detection and Quantification, 2014 - Elsevier
PCR is a formidable and potent technology that serves as an indispensable tool in a wide
range of biological disciplines. However, due to the ease of use and often lack of rigorous …

Reliable, verifiable and efficient monitoring of biodiversity via metabarcoding

Y Ji, L Ashton, SM Pedley, DP Edwards, Y Tang… - Ecology …, 2013 - Wiley Online Library
To manage and conserve biodiversity, one must know what is being lost, where, and why, as
well as which remedies are likely to be most effective. Metabarcoding technology can …

Biodiversity soup: metabarcoding of arthropods for rapid biodiversity assessment and biomonitoring

DW Yu, Y Ji, BC Emerson, X Wang, C Ye… - Methods in Ecology …, 2012 - Wiley Online Library
Traditional biodiversity assessment is costly in time, money and taxonomic expertise.
Moreover, data are frequently collected in ways (eg visual bird lists) that are unsuitable for …

[HTML][HTML] Detection of SARS-CoV-2 intra-host recombination during superinfection with Alpha and Epsilon variants in New York City

JO Wertheim, JC Wang, M Leelawong… - Nature …, 2022 - nature.com
Recombination is an evolutionary process by which many pathogens generate diversity and
acquire novel functions. Although a common occurrence during coronavirus replication …

[HTML][HTML] Rapid and adaptive evolution of MHC genes under parasite selection in experimental vertebrate populations

C Eizaguirre, TL Lenz, M Kalbe, M Milinski - Nature communications, 2012 - nature.com
The genes of the major histocompatibility complex are the most polymorphic genes in
vertebrates, with more than 1,000 alleles described in human populations. How this …

[HTML][HTML] A 454 multiplex sequencing method for rapid and reliable genotyping of highly polymorphic genes in large-scale studies

M Galan, E Guivier, G Caraux, N Charbonnel… - BMC genomics, 2010 - Springer
Background High-throughput sequencing technologies offer new perspectives for
biomedical, agronomical and evolutionary research. Promising progresses now concern the …

MHC‐based mate choice combines good genes and maintenance of MHC polymorphism

C Eizaguirre, SE Yeates, TL Lenz, M Kalbe… - Molecular …, 2009 - Wiley Online Library
Polymorphic genes of the major histocompatibility complex (MHC) are regarded as essential
genes for individual fitness under conditions of natural and sexual selection. To test this …

Reducing the impact of PCR-mediated recombination in molecular evolution and environmental studies using a new-generation high-fidelity DNA polymerase

DJG Lahr, LA Katz - Biotechniques, 2009 - Future Science
PCR-mediated recombination can greatly impact estimates of diversity, both in
environmental studies and in analyses of gene family evolution. Here we measure chimera …

Methods for MHC genotyping in non‐model vertebrates

W Babik - Molecular ecology resources, 2010 - Wiley Online Library
Genes of the major histocompatibility complex (MHC) are considered a paradigm of
adaptive evolution at the molecular level and as such are frequently investigated by …