Reverse Genetics Systems for Emerging and Re-Emerging Swine Coronaviruses and Applications

H Jiang, T Wang, L Kong, B Li, Q Peng - Viruses, 2023 - mdpi.com
Emerging and re-emerging swine coronaviruses (CoVs), including porcine epidemic
diarrhea virus (PEDV), porcine deltacoronavirus (PDCoV), and swine acute diarrhea …

Reprogramming viral immune evasion for a rational design of next-generation vaccines for RNA viruses

CM Su, Y Du, RRR Rowland, Q Wang… - Frontiers in …, 2023 - frontiersin.org
Type I interferons (IFNs-α/β) are antiviral cytokines that constitute the innate immunity of
hosts to fight against viral infections. Recent studies, however, have revealed the pleiotropic …

Deletion of a 7-amino-acid region in the porcine epidemic diarrhea virus envelope protein induces higher type I and III interferon responses and results in attenuation …

Z Li, Z Ma, W Han, C Chang, Y Li, X Guo… - Journal of …, 2023 - Am Soc Microbiol
Porcine epidemic diarrhea virus (PEDV) leads to enormous economic losses for the pork
industry. However, the commercial vaccines failed to fully protect against the epidemic …

Isolation, identification, and pathogenicity of porcine epidemic diarrhea virus

Y Sun, T Gong, D Wu, Y Feng, Q Gao, J Xing… - Frontiers in …, 2023 - frontiersin.org
Porcine epidemic diarrhea (PED) is an enterophilic infectious disease caused by the porcine
epidemic diarrhea virus (PEDV), which can lead to dehydration-like diarrhea in piglets with a …

PEDV evades MHC-I-related immunity through nsp1-mediated NLRC5 translation inhibition

X Liu, M Zhang, L Yin, L Kang, Y Luo, X Wang… - Journal of …, 2024 - journals.asm.org
Major histocompatibility complex class I (MHC-I) plays crucial roles against viral infections
not only by initiating CD8+ T cell immunity but also by modulating natural killer (NK) cell …

Development of a safe and broad-spectrum attenuated PEDV vaccine candidate by S2 subunit replacement

D Zhang, Y Xie, Q Liao, Z Jiao, R Liang… - Journal of …, 2024 - journals.asm.org
Porcine epidemic diarrhea (PED) has caused serious economic losses to the swine
livestock industry. Due to the rapid variation in the PEDV) genome, especially the spike (S) …

SADS-CoV nsp1 inhibits the STAT1 phosphorylation by promoting K11/K48-linked polyubiquitination of JAK1 and blocks the STAT1 acetylation by degrading CBP

Y Xiang, C Mou, L Zhu, Z Wang, K Shi, W Bao… - Journal of Biological …, 2024 - ASBMB
The newly discovered zoonotic coronavirus swine acute diarrhea syndrome coronavirus
(SADS-CoV) causes acute diarrhea, vomiting, dehydration, and high mortality rates in …

Progress of research on coronaviruses and toroviruses in large domestic animals using reverse genetics systems

M Ujike, T Suzuki - The Veterinary Journal, 2024 - Elsevier
The generation of genetically engineered recombinant viruses from modified DNA/RNA is
commonly referred to as reverse genetics, which allows the introduction of desired mutations …

Developing Next-Generation Live Attenuated Vaccines for Porcine Epidemic Diarrhea Using Reverse Genetic Techniques

R Yu, S Dong, B Chen, F Si, C Li - Vaccines, 2024 - mdpi.com
Porcine epidemic diarrhea virus (PEDV) is the etiology of porcine epidemic diarrhea (PED),
a highly contagious digestive disease in pigs and especially in neonatal piglets, in which a …

A Comprehensive View on the Protein Functions of Porcine Epidemic Diarrhea Virus

X Li, Y Wu, Z Yan, G Li, J Luo, S Huang, X Guo - Genes, 2024 - mdpi.com
Porcine epidemic diarrhea (PED) virus (PEDV) is one of the main pathogens causing
diarrhea in piglets and fattening pigs. The clinical signs of PED are vomiting, acute diarrhea …