Cell-free gene expression: methods and applications
Cell-free gene expression (CFE) systems empower synthetic biologists to build biological
molecules and processes outside of living intact cells. The foundational principle is that …
molecules and processes outside of living intact cells. The foundational principle is that …
Cell-free protein synthesis in micro compartments: building a minimal cell from biobricks
The construction of a minimal cell that exhibits the essential characteristics of life is a great
challenge in the field of synthetic biology. Assembling a minimal cell requires …
challenge in the field of synthetic biology. Assembling a minimal cell requires …
Transcription and translation in cytomimetic protocells perform most efficiently at distinct macromolecular crowding conditions
MA Vibhute, MH Schaap, RJM Maas… - ACS Synthetic …, 2020 - ACS Publications
The formation of cytomimetic protocells that capture the physicochemical aspects of living
cells is an important goal in bottom-up synthetic biology. Here, we recreated the crowded …
cells is an important goal in bottom-up synthetic biology. Here, we recreated the crowded …
[HTML][HTML] Cell-free microcompartmentalised transcription–translation for the prototyping of synthetic communication networks
E Dubuc, PA Pieters, AJ van der Linden… - Current opinion in …, 2019 - Elsevier
Highlights•Cell-free transcription and translation systems are used for testing and facilitating
the implementation of novel circuits in cells.•Cell-free transcription and translation systems …
the implementation of novel circuits in cells.•Cell-free transcription and translation systems …
Protein‐Encoding Free‐Standing RNA Hydrogel for Sub‐Compartmentalized Translation
RNA can self‐fold into complex structures that can serve as major biological regulators in
protein synthesis and in catalysis. Due to the abundance of structural primitives and …
protein synthesis and in catalysis. Due to the abundance of structural primitives and …
Emerging advances of cell‐free systems toward artificial cells
Y Zhu, X Guo, J Liu, F Li, D Yang - Small Methods, 2020 - Wiley Online Library
Artificial cells that mimic the architectural and functional characteristics of living cells not only
shed light on the physical principle of life but also facilitate development in the areas such as …
shed light on the physical principle of life but also facilitate development in the areas such as …
Noise minimization in cell-free gene expression
MW Bartelds, Ó García-Blay, PGA Verhagen… - ACS Synthetic …, 2023 - ACS Publications
Biochemical reactions that involve small numbers of molecules are accompanied by a
degree of inherent randomness that results in noisy reaction outcomes. In synthetic biology …
degree of inherent randomness that results in noisy reaction outcomes. In synthetic biology …
Bottom-up synthetic biology using cell-free protein synthesis
K Yue, Y Li, M Cao, L Shen, J Gu, L Kai - Cell-free Macromolecular …, 2023 - Springer
Technical advances in biotechnology have greatly accelerated the development of bottom-
up synthetic biology. Unlike top-down approaches, bottom-up synthetic biology focuses on …
up synthetic biology. Unlike top-down approaches, bottom-up synthetic biology focuses on …
A sustainable and efficient artificial microgel system: toward creating a configurable synthetic cell
Artificial cells are a powerful platform in the study of synthetic biology and other valuable
fields. They share a great potential in defining and utilizing the superiority of the living …
fields. They share a great potential in defining and utilizing the superiority of the living …
Cell‐Free Protein Synthesis in Bifunctional Hyaluronan Microgels: A Strategy for In Situ Immobilization and Purification of His‐Tagged Proteins
T Heida, T Köhler, A Kaufmann, MJ Männel… - …, 2020 - Wiley Online Library
In current cell‐free biotechnology, mimicking physicochemical aspects of cellular life while
providing efficient protein synthesis, separation, and purification is a crucial step towards …
providing efficient protein synthesis, separation, and purification is a crucial step towards …