Tools and methods for high-throughput single-cell imaging with the mother machine

R Thiermann, M Sandler, G Ahir, JT Sauls, J Schroeder… - Elife, 2024 - elifesciences.org
Despite much progress, image processing remains a significant bottleneck for high-
throughput analysis of microscopy data. One popular platform for single-cell time-lapse …

Single‐cell segmentation in bacterial biofilms with an optimized deep learning method enables tracking of cell lineages and measurements of growth rates

E Jelli, T Ohmura, N Netter, M Abt… - Molecular …, 2023 - Wiley Online Library
Bacteria often grow into matrix‐encased three‐dimensional (3D) biofilm communities, which
can be imaged at cellular resolution using confocal microscopy. From these 3D images …

[HTML][HTML] Structural and functional analyses of nematode-derived antimicrobial peptides support the occurrence of direct mechanisms of worm-microbiota interactions

J Rooney, E Rivera-de-Torre, R Li, K Mclean… - Computational and …, 2024 - Elsevier
The complex relationships between gastrointestinal (GI) nematodes and the host gut
microbiota have been implicated in key aspects of helminth disease and infection outcomes …

Quantitative microbiology with widefield microscopy: navigating optical artefacts for accurate interpretations

G Hardo, R Li, S Bakshi - npj Imaging, 2024 - nature.com
Time-resolved live-cell imaging using widefield microscopy is instrumental in quantitative
microbiology research. It allows researchers to track and measure the size, shape, and …

[HTML][HTML] A general algorithm for consensus 3D cell segmentation from 2D segmented stacks

FY Zhou, C Yapp, Z Shang, S Daetwyler, Z Marin… - bioRxiv, 2024 - ncbi.nlm.nih.gov
Cell segmentation is the fundamental task. Only by segmenting, can we define the
quantitative spatial unit for collecting measurements to draw biological conclusions. Deep …

Segmentation of dense and multi-species bacterial colonies achieved using models trained on synthetic microscopy images

V Hickl, A Khan, RM Rossi, K Maniura-Weber… - arXiv preprint arXiv …, 2024 - arxiv.org
The spread of microbial infections is governed by the self-organization of bacteria on
surfaces. Limitations of live imaging techniques make collective behaviors in clinically …

Single-cell imaging of the lytic phage life cycle dissects the timing and noise in individual infection steps

C Wedd, T Yunusov, A Smith, R Li, G Hardo, M Hunter… - bioRxiv, 2024 - biorxiv.org
When a lytic bacteriophage infects a cell, it hijacks the cell's resources to replicate itself,
ultimately causing cell lysis and the release of new virions. As phages function as obligate …

2024 World Scientific Publishing Company

TL from Synthetic, A Milias-Argeitis… - Frontiers In Bioimage …, 2024 - books.google.com
In recent years, deep neural networks for cell segmentation have achieved impressive
advances over more traditional computer vision methods. However, generating large …

Transfer Learning from Synthetic Data for Cell Segmentation and Tracking

A Milias-Argeitis, H Kruitbosch - Frontiers in Bioimage Informatics …, 2024 - World Scientific
In recent years, deep neural networks for cell segmentation have achieved impressive
advances over more traditional computer vision methods. However, generating large …

Tools and methods for high-throughput single-cell imaging with the mother machine.

M Sandler, G Ahir, J Sauls, J Schroeder, S Brown… - 2024 - escholarship.org
Despite much progress, image processing remains a significant bottleneck for high-
throughput analysis of microscopy data. One popular platform for single-cell time-lapse …