Recent developments in synthetic biology may bring the bottom-up generation of a synthetic
cell within reach. A key feature of a living synthetic cell is a functional cell cycle, in which …

DNA polymerases are enzymes capable of synthesizing DNA. They are involved in
replication of genomes of all cellular organisms as well as in processes of DNA repair and …

Replication, heredity, and evolution are characteristic of Life. We and others have postulated
that the reconstruction of a synthetic living system in the laboratory will be contingent on the …

The in vitro reconstruction of life-like self-reproducing systems is a major challenge in in vitro
synthetic biology. Self-reproduction requires regeneration of all molecules involved in DNA …

The bottom-up construction of bio-inspired systems capable of self-maintenance and
reproduction is a central goal in systems chemistry and synthetic biology. A particular …

Single-cell genomic whole genome amplification (WGA) is a crucial step in single-cell
sequencing, yet its low amplification efficiency, incomplete and uneven genome …

Cell extract-based cell-free gene expression (CFE) systems break cellular boundaries and
create emerging platforms for synthetic biology applications. However, these systems lack …

Replication, heredity, and evolution are characteristic of Life. We and others have postulated
that the reconstruction of a synthetic living system in the laboratory will be contingent on the …

The use of cell-free production systems in droplet microfluidic devices has gained significant
interest during the last decade. Encapsulating DNA replication, RNA transcription, and …

Riboswitches have recently attracted the attention of synthetic biologists as an alternative to
transcription factors for genetic regulation because of their engineerability, relative simplicity …