We describe the first systematic study of a family of inteins, the split DnaE inteins from
cyanobacteria. By measuring in vivo splicing efficiencies and in vitro kinetics, we …

Chemical synthesis is a well‐established method for the preparation in the research
laboratory of multiple‐tens‐of‐milligram amounts of correctly folded, high purity protein …

Unparalleled in size and scope, this new major reference integrates academic and industrial
knowledge into a single resource, allowing for a unique overview of the entire field. Adopting …

Intein‐mediated protein splicing is a self‐catalytic process in which the intervening intein
sequence is removed from a precursor protein and the flanking extein segments are ligated …

Disclosed herein are split inteins, fused proteins of split inteins, and methods of using split
inteins to efficiently purify and modify proteins of interest. Thus, provided herein are fusion …

Inteins are single turnover enzymes that splice out of protein precursors during maturation of
the host protein (extein). The Cys or Ser at the N terminus of most inteins initiates a four-step …

While the model bacteria Escherichia coli and Bacillus subtilis harbor single chromosomes,
which is known as monoploidy, some freshwater cyanobacteria contain multiple …

For the proteins that cannot be expressed exactly by cell expression technology (eg,
proteins with multiple posttranslational modifications or toxic proteins), chemical synthesis is …

The purification of recombinant proteins is a complicated process that requires a thorough
understanding of the physical and chemical properties of each protein of interest. The …

Nuclear magnetic resonance (NMR) spectroscopy has emerged as one of the principle
techniques of structural biology. It is not only a powerful method for elucidating the three …