Although messenger RNA (mRNA) has proved effective as a vaccine, its potential as a
general therapeutic modality is limited by its instability and low translation capacity. To …

The present study focused on the design and synthesis of covalent DNA dendrons bearing
multivalent cytosine‐phosphate‐guanine oligodeoxynucleotides (CpG ODNs) that can …

The enzymatic processing of cellular RNA molecules requires selective recognition of
unique chemical and topological features. The unusual 2′, 5′-phosphodiester linkages in …

Turnover of the branched RNA intermediates and products of pre-mRNA splicing is
mediated by the lariat-debranching enzyme Dbr1. We characterized a homolog of Dbr1 from …

Intron lariats are circular, branched RNAs (bRNAs) produced during pre-mRNA splicing.
Their unusual chemical and topological properties arise from branch-point nucleotides …

Tpt1 catalyzes the transfer of an internal 2′-monophosphate moiety (2′-PO4) from a
“branched” 2′-PO4 RNA splice junction to NAD+ to form a “clean” 2′-OH, 3′-5 …

Although group II intron ribozymes are intensively studied the question how structural
dynamics affects splicing catalysis has remained elusive. We report for the first time that the …

Branching points in DNA nanostructures are usually 3-or 4-way junctions maintained by
Watson-Crick non-covalent interactions. However, covalently bound DNA stars could …

In eukaryotic cells, the introns are excised from pre-mRNA by the spliceosome. These
introns typically have a lariat configuration due to the 2′-5′ phosphodiester bond between …

Alternative splicing is a powerful mechanism present in eukaryotic cells to obtain a wide
range of transcripts and protein isoforms from a relatively small number of genes. The …