Maturing glycoproteomics technologies provide unique structural insights into the N-glycoproteome and its regulation in health and disease

M Thaysen-Andersen, NH Packer, BL Schulz - Molecular & Cellular …, 2016 - ASBMB
The glycoproteome remains severely understudied because of significant analytical
challenges associated with glycoproteomics, the system-wide analysis of intact …

Recent advances in analytical approaches for glycan and glycopeptide quantitation

DG Delafield, L Li - Molecular & Cellular Proteomics, 2021 - ASBMB
Growing implications of glycosylation in physiological occurrences and human disease have
prompted intensive focus on revealing glycomic perturbations through absolute and relative …

Chemical tagging in mass spectrometry for systems biology

T Huang, MR Armbruster, JB Coulton… - Analytical …, 2018 - ACS Publications
Mass spectrometry (MS) offers unparalleled qualitative and quantitative chemical
information. While increasingly considered a universal detector, the limitations of MS to …

Deciphering Protein O-Glycosylation: Solid-Phase Chemoenzymatic Cleavage and Enrichment

S Yang, P Onigman, WW Wu, J Sjogren… - Analytical …, 2018 - ACS Publications
Glycosylation plays a critical role in the biosynthetic-secretory pathway in the endoplasmic
reticulum (ER) and Golgi apparatus. Over 50% of mammalian cellular proteins are typically …

The neuraminidase of A (H3N2) influenza viruses circulating since 2016 is antigenically distinct from the A/Hong Kong/4801/2014 vaccine strain

H Wan, J Gao, H Yang, S Yang, R Harvey… - Nature …, 2019 - nature.com
Abstract A (H3N2) virus predominated recent influenza seasons, which has resulted in the
rigorous investigation of haemagglutinin, but whether neuraminidase (NA) has undergone …

Data-independent acquisition-based mass spectrometry (DIA-MS) for quantitative analysis of intact N-linked glycopeptides

M Dong, TSM Lih, M Ao, Y Hu, SY Chen… - Analytical …, 2021 - ACS Publications
N-linked protein glycosylation is a key regulator in various biological functions. Previous
studies have shown that aberrant glycosylation is associated with many diseases. Therefore …

Simultaneous quantification of N-and O-glycans using a solid-phase method

S Yang, Y Hu, L Sokoll, H Zhang - Nature protocols, 2017 - nature.com
Glycosylation has a pivotal role in a diverse range of biological activities, modulating the
structure and function of proteins. Glycogens coupled to the nitrogen atom (N-linked) of …

QUANTITY: an isobaric tag for quantitative glycomics

S Yang, M Wang, L Chen, B Yin, G Song, IV Turko… - Scientific reports, 2015 - nature.com
Glycan is an important class of macromolecules that play numerous biological functions.
Quantitative glycomics-analysis of glycans at global level-however, is far behind genomics …

Identification of sialic acid linkages on intact glycopeptides via differential chemical modification using IntactGIG-HILIC

S Yang, WW Wu, RF Shen, M Bern… - Journal of the American …, 2018 - ACS Publications
Mass spectrometric analysis of intact glycopeptides can reveal detailed information about
glycosite, glycan structural features, and their heterogeneity. Sialyl glycopeptides can be …

Strategies for proteome-wide quantification of glycosylation macro-and micro-heterogeneity

P Fang, Y Ji, T Oellerich, H Urlaub, KT Pan - International journal of …, 2022 - mdpi.com
Protein glycosylation governs key physiological and pathological processes in human cells.
Aberrant glycosylation is thus closely associated with disease progression. Mass …