mNGS in clinical microbiology laboratories: on the road to maturity

D Han, Z Li, R Li, P Tan, R Zhang… - Critical reviews in …, 2019 - Taylor & Francis
Metagenomic next-generation sequencing (mNGS) is increasingly being applied in clinical
laboratories for unbiased culture-independent diagnosis. Whether it can be a next routine …

High‐throughput metagenomics for identification of pathogens in the clinical settings

N Li, Q Cai, Q Miao, Z Song, Y Fang, B Hu - Small methods, 2021 - Wiley Online Library
The application of sequencing technology is shifting from research to clinical laboratories
owing to rapid technological developments and substantially reduced costs. However …

[HTML][HTML] Metagenomics next-generation sequencing tests take the stage in the diagnosis of lower respiratory tract infections

Z Diao, D Han, R Zhang, J Li - Journal of advanced research, 2022 - Elsevier
Background Metagenomic next-generation sequencing (mNGS) has changed the diagnosis
landscape of lower respiratory tract infections (LRIs). With the development of newer …

Evaluation of NGS-based approaches for SARS-CoV-2 whole genome characterisation

C Charre, C Ginevra, M Sabatier, H Regue… - Virus …, 2020 - academic.oup.com
Since the beginning of the COVID-19 outbreak, SARS-CoV-2 whole-genome sequencing
(WGS) has been performed at unprecedented rate worldwide with the use of very diverse …

[HTML][HTML] Re-emergence of enterovirus D68 in Europe after easing the COVID-19 lockdown, September 2021

KSM Benschop, J Albert, A Anton, C Andrés… - …, 2021 - eurosurveillance.org
We report a rapid increase in enterovirus D68 (EV-D68) infections, with 139 cases reported
from eight European countries between 31 July and 14 October 2021. This upsurge is in line …

[HTML][HTML] Metagenomic sequencing for microbial DNA in human samples: emerging technological advances

Y Shi, G Wang, HCH Lau, J Yu - International journal of molecular …, 2022 - mdpi.com
Whole genome metagenomic sequencing is a powerful platform enabling the simultaneous
identification of all genes from entirely different kingdoms of organisms in a complex sample …

Undetectable viral RNA in oocytes from SARS-CoV-2 positive women

M Barragan, JJ Guillén, N Martin-Palomino… - Human …, 2021 - academic.oup.com
A central concern for the safe provision of ART during the current coronavirus disease 2019
(COVID-19) pandemic is the possibility of vertical transmission of severe acute respiratory …

[PDF][PDF] Molecular characterization of SARS-CoV-2 in the first COVID-19 cluster in France reveals an amino acid deletion in nsp2 (Asp268del)

A Bal, G Destras, A Gaymard… - Clinical Microbiology …, 2020 - Elsevier
[3] Corman VM, Landt O, Kaiser M, Molenkamp R, Meijer A, Chu DKW, et al. Detection of
103 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR. Euro Surveill 2020; 25. 104 …

[HTML][HTML] Retrospective validation of a metagenomic sequencing protocol for combined detection of RNA and DNA viruses using respiratory samples from pediatric …

S van Boheemen, AL van Rijn, N Pappas… - The Journal of Molecular …, 2020 - Elsevier
Viruses are the main cause of respiratory tract infections. Metagenomic next-generation
sequencing (mNGS) enables unbiased detection of all potential pathogens. To apply mNGS …

[HTML][HTML] High throughput sequencing for the detection and characterization of RNA viruses

AH Fitzpatrick, A Rupnik, H O'Shea, F Crispie… - Frontiers in …, 2021 - frontiersin.org
This review aims to assess and recommend approaches for targeted and agnostic High
Throughput Sequencing of RNA viruses in a variety of sample matrices. HTS also referred to …