Bacterial ghosts-based vaccine and drug delivery systems

H Chen, H Ji, X Kong, P Lei, Q Yang, W Wu, L Jin… - Pharmaceutics, 2021 - mdpi.com
Bacterial ghosts (BGs) are empty bacterial envelopes of Gram-negative bacteria produced
by controlled expressions of cloned gene E, forming a lysis tunnel structure within the …

[HTML][HTML] Recent advances to accelerate purification process development: A review with a focus on vaccines

D Keulen, G Geldhof, O Le Bussy, M Pabst… - … of Chromatography A, 2022 - Elsevier
The safety requirements for vaccines are extremely high since they are administered to
healthy people. For that reason, vaccine development is time-consuming and very …

Interface-based design of high-affinity affibody ligands for the purification of RBD from spike proteins

S Song, Q Shi - Molecules, 2023 - mdpi.com
The outbreak of coronavirus disease 2019 (COVID-19) has sparked an urgent demand for
advanced diagnosis and vaccination worldwide. The discovery of high-affinity ligands is of …

High‐Yield Separation of Extracellular Vesicles Using Programmable Zwitterionic Coacervates

C Paganini, U Capasso Palmiero, S Picciotto… - Small, 2023 - Wiley Online Library
Programmable coacervates based on zwitterionic polymers are designed as dynamic
materials for ion exchange bioseparation. These coacervates are proposed as promising …

Pressure‐dependent fouling behavior during sterile filtration of mRNA‐containing lipid nanoparticles

KO Messerian, A Zverev… - Biotechnology and …, 2022 - Wiley Online Library
The COVID‐19 pandemic has generated growing interest in the development of mRNA‐
based vaccines and therapeutics. However, the size and properties of the lipid nanoparticles …

Regulation on both pore structure and pressure-resistant property of uniform agarose microspheres for high-resolution chromatography

L Zhao, X Che, Y Huang, K Zhu, Y Du, J Gao… - … of Chromatography A, 2022 - Elsevier
How to improve the performance of chromatographic media is very important in
chromatography. Uniform agarose microspheres were successfully prepared using …

Purification processes of live virus vaccine candidates expressed in adherent Vero cell lines via multimodal chromatography in flowthrough mode

S Konstantinidis, MR Poplyk, W Ma… - Biotechnology and …, 2024 - Wiley Online Library
Live virus vaccine (LVV) purification, employing chromatography, can be challenged by low
binding capacities and elution yields. Alternatively, processes relying solely on enzymatic …

Development of versatile affinity‐based system for one step purification process: Case of Group A Streptococcus vaccine

A Chevrel, L Candela, E Innocenti… - Biotechnology and …, 2022 - Wiley Online Library
Affinity capture is one of the most attractive strategies for simplifying downstream processing.
Although it is a key mainstream approach for antibody purification, the same is not true for …

Discovery of broad-spectrum high-affinity peptide ligands of spike protein for the vaccine purification of SARS-CoV-2 and Omicron variants

J Ma, Y Huang, G Jia, X Dong, Q Shi, Y Sun - International Journal of …, 2024 - Elsevier
To combat with emerging SARS-CoV-2 variants of concern (VOCs), we report the
identification of a set of unique HWK-motif peptide ligands for the receptor-binding domain …

Affinity chromatography for virus-like particle manufacturing: challenges, solutions, and perspectives

J Ma, Z Tian, Q Shi, X Dong, Y Sun - Journal of Chromatography A, 2024 - Elsevier
The increasing medical application of virus-like particles (VLPs), notably vaccines and viral
vectors, has increased the demand for commercial VLP production. However, VLP …