Insertion sequences (ISs), arguably the smallest and most numerous autonomous
transposable elements (TEs), are important players in shaping their host genomes. This …

Nucleases cleave the phosphodiester bonds of nucleic acids and may be endo or exo,
DNase or RNase, topoisomerases, recombinases, ribozymes, or RNA splicing enzymes. In …

Transposition has a key role in reshaping genomes of all living organisms. Insertion
sequences of IS200/IS605 and IS607 families are among the simplest mobile genetic …

CRISPR-Cas systems provide microbes with adaptive immunity to infectious nucleic acids
and are widely employed as genome editing tools. These tools use RNA-guided Cas …

Genomic rearrangements, encompassing mutational changes in the genome such as
insertions, deletions or inversions, are essential for genetic diversity. These rearrangements …

CRISPR-Cas nucleases are powerful tools for manipulating nucleic acids; however, targeted
insertion of DNA remains a challenge, as it requires host cell repair machinery. Here we …

As the evolutionary ancestor of Cas12 nuclease, the transposon (IS200/IS605)-encoded
TnpB proteins act as compact RNA-guided DNA endonucleases. To explore their …

Insertion sequences are compact and pervasive transposable elements found in bacteria,
which encode only the genes necessary for their mobilization and maintenance. IS200-and …

HUH endonucleases are numerous and widespread in all three domains of life. The major
function of these enzymes is processing a range of mobile genetic elements by catalysing …

We have divided this review into two major sections. In one, we have attempted to present
an overview of our current understanding of prokaryotic insertion sequences (IS), their …