Applying a high voltage to a liquid passing through a capillary disintegrates the liquid
meniscus to generate a fine spray of charged droplets. As the droplets evaporate, polar …

A single gene yields many forms of proteins via combinations of posttranscriptional/
posttranslational modifications. Proteins also fold into higher‐order structures and interact …

Variable-temperature electrospray ionization (vT-ESI) native mass spectrometry (nMS) is
used to determine the thermodynamics for stepwise binding of up to 14 ATP molecules to …

Native mass spectrometry is a powerful tool for the analysis of noncovalent complexes.
When coupled with high-resolution ion mobility, this technique can be used to investigate …

Electron capture dissociation (ECD) is now a well-established method for sequencing
peptides and performing top-down analysis on proteins of less than 30 kDa, and there is …

Charge reduction reactions are important for native mass spectrometry (nMS) because lower
charge states help retain native-like conformations and preserve noncovalent interactions of …

Digital mass filters are advantageous for the analysis of large molecules due to the ability to
perform ion isolation of high-m/z ions without the generation of very high radio frequency …

Protein complexes are essential for proteins' folding and biological function. Currently,
native analysis of large multimeric protein complexes remains challenging. Structural …

Iron–sulfur (Fe–S) cluster (ISC) cofactors are required for the function of many critical cellular
processes. In the ISC Fe–S cluster biosynthetic pathway, IscU assembles Fe–S cluster …

Homo‐oligomeric ligand‐activated proteins are ubiquitous in biology. The functions of such
molecules are commonly regulated by allosteric coupling between ligand‐binding sites …