Bottom-up proteomics: advancements in sample preparation

VA Duong, H Lee - International journal of molecular sciences, 2023 - mdpi.com
Liquid chromatography–tandem mass spectrometry (LC–MS/MS)-based proteomics is a
powerful technique for profiling proteomes of cells, tissues, and body fluids. Typical bottom …

RNA interactome capture in Escherichia coli globally identifies RNA-binding proteins

TS Stenum, AD Kumar, FA Sandbaumhüter… - Nucleic Acids …, 2023 - academic.oup.com
RNA-binding proteins (RPBs) are deeply involved in fundamental cellular processes in
bacteria and are vital for their survival. Despite this, few studies have so far been dedicated …

Alkaline-SDS cell lysis of microbes with acetone protein precipitation for proteomic sample preparation in 96-well plate format

Y Chen, JW Gin, Y Wang, M de Raad, S Tan… - PloS one, 2023 - journals.plos.org
Plate-based proteomic sample preparation offers a solution to the large sample throughput
demands in the biotechnology field where hundreds or thousands of engineered microbes …

Label-free quantitative thermal proteome profiling reveals target transcription factors with activities modulated by MC3R signaling

FA Sandbaumhüter, M Nezhyva, PE Andrén… - Analytical …, 2023 - ACS Publications
Thermal proteome profiling with label-free quantitation using ion-mobility-enhanced LC–MS
offers versatile data sets, providing information on protein differential expression, thermal …

Coupling Microdroplet-Based Sample Preparation, Multiplexed Isobaric Labeling, and Nanoflow Peptide Fractionation for Deep Proteome Profiling of the Tissue …

M Veličković, TL Fillmore, IK Attah, C Posso… - Analytical …, 2024 - ACS Publications
There is increasing interest in developing in-depth proteomic approaches for mapping tissue
heterogeneity in a cell-type-specific manner to better understand and predict the function of …

Zero-degree Celsius capillary electrophoresis electrospray ionization for hydrogen exchange mass spectrometry

JT Aerts, PE Andrén, ET Jansson - Analytical Chemistry, 2022 - ACS Publications
Currently, fast liquid chromatographic separations at low temperatures are exclusively used
for the separation of peptides generated in hydrogen deuterium exchange (HDX) workflows …

Evaluation of PAC and FASP Performance: DIA-Based Quantitative Proteomic Analysis

MS Murfuni, LE Prestagiacomo, A Giuliano… - International Journal of …, 2024 - mdpi.com
The aim of this study was to compare filter-aided sample preparation (FASP) and protein
aggregation capture (PAC) starting from a three-species protein mix (Human, Soybean and …

Inquiry-based Learning of Proteomics and Metabolomics

M Nezhyva, JT Aerts… - Journal of Chemical …, 2024 - ACS Publications
Given the rapid development of data-driven experimental procedures within the life
sciences, it is important to equip students with proper skills and knowledge on how to obtain …

Integrative Approaches in Shotgun Proteomics: From sample preparation to multifaceted data analysis

M Nezhyva - 2024 - diva-portal.org
Integrative Approaches in Shotgun Proteomics : From sample preparation to multifaceted
data analysis diva-portal.org Digitala Vetenskapliga Arkivet Simple search Advanced …

Novel LC-MS Strategies for Enhancing Understanding of Drug Effects and Exploring Disease Mechanisms

X Zhu - 2024 - search.proquest.com
Abstract Liquid Chromatography-Mass Spectrometry (LC-MS)-based techniques are widely
employed in the pharmaceutical research to support drug development from the discovery to …