Bottom-up proteomics: advancements in sample preparation
Liquid chromatography–tandem mass spectrometry (LC–MS/MS)-based proteomics is a
powerful technique for profiling proteomes of cells, tissues, and body fluids. Typical bottom …
powerful technique for profiling proteomes of cells, tissues, and body fluids. Typical bottom …
RNA interactome capture in Escherichia coli globally identifies RNA-binding proteins
TS Stenum, AD Kumar, FA Sandbaumhüter… - Nucleic Acids …, 2023 - academic.oup.com
RNA-binding proteins (RPBs) are deeply involved in fundamental cellular processes in
bacteria and are vital for their survival. Despite this, few studies have so far been dedicated …
bacteria and are vital for their survival. Despite this, few studies have so far been dedicated …
Alkaline-SDS cell lysis of microbes with acetone protein precipitation for proteomic sample preparation in 96-well plate format
Y Chen, JW Gin, Y Wang, M de Raad, S Tan… - PloS one, 2023 - journals.plos.org
Plate-based proteomic sample preparation offers a solution to the large sample throughput
demands in the biotechnology field where hundreds or thousands of engineered microbes …
demands in the biotechnology field where hundreds or thousands of engineered microbes …
Label-free quantitative thermal proteome profiling reveals target transcription factors with activities modulated by MC3R signaling
FA Sandbaumhüter, M Nezhyva, PE Andrén… - Analytical …, 2023 - ACS Publications
Thermal proteome profiling with label-free quantitation using ion-mobility-enhanced LC–MS
offers versatile data sets, providing information on protein differential expression, thermal …
offers versatile data sets, providing information on protein differential expression, thermal …
Coupling Microdroplet-Based Sample Preparation, Multiplexed Isobaric Labeling, and Nanoflow Peptide Fractionation for Deep Proteome Profiling of the Tissue …
M Veličković, TL Fillmore, IK Attah, C Posso… - Analytical …, 2024 - ACS Publications
There is increasing interest in developing in-depth proteomic approaches for mapping tissue
heterogeneity in a cell-type-specific manner to better understand and predict the function of …
heterogeneity in a cell-type-specific manner to better understand and predict the function of …
Zero-degree Celsius capillary electrophoresis electrospray ionization for hydrogen exchange mass spectrometry
Currently, fast liquid chromatographic separations at low temperatures are exclusively used
for the separation of peptides generated in hydrogen deuterium exchange (HDX) workflows …
for the separation of peptides generated in hydrogen deuterium exchange (HDX) workflows …
Evaluation of PAC and FASP Performance: DIA-Based Quantitative Proteomic Analysis
MS Murfuni, LE Prestagiacomo, A Giuliano… - International Journal of …, 2024 - mdpi.com
The aim of this study was to compare filter-aided sample preparation (FASP) and protein
aggregation capture (PAC) starting from a three-species protein mix (Human, Soybean and …
aggregation capture (PAC) starting from a three-species protein mix (Human, Soybean and …
Inquiry-based Learning of Proteomics and Metabolomics
M Nezhyva, JT Aerts… - Journal of Chemical …, 2024 - ACS Publications
Given the rapid development of data-driven experimental procedures within the life
sciences, it is important to equip students with proper skills and knowledge on how to obtain …
sciences, it is important to equip students with proper skills and knowledge on how to obtain …
Integrative Approaches in Shotgun Proteomics: From sample preparation to multifaceted data analysis
M Nezhyva - 2024 - diva-portal.org
Integrative Approaches in Shotgun Proteomics : From sample preparation to multifaceted
data analysis diva-portal.org Digitala Vetenskapliga Arkivet Simple search Advanced …
data analysis diva-portal.org Digitala Vetenskapliga Arkivet Simple search Advanced …
Novel LC-MS Strategies for Enhancing Understanding of Drug Effects and Exploring Disease Mechanisms
X Zhu - 2024 - search.proquest.com
Abstract Liquid Chromatography-Mass Spectrometry (LC-MS)-based techniques are widely
employed in the pharmaceutical research to support drug development from the discovery to …
employed in the pharmaceutical research to support drug development from the discovery to …