Most methods for the analysis of oligosaccharides from biological sources require a glycan
derivatization step: glycans may be derivatized to introduce a chromophore or fluorophore …

1.1. General Considerations Structural intricacies of carbohydrate molecules and their
propensity to form varied linkages, substitutions, and branching patterns have fascinated …

This protocol shows how to obtain a detailed glycan compositional and structural profile from
purified glycoproteins or protein mixtures, and it can be used to distinguish different isobaric …

Human milk oligosaccharides (HMOs) perform a number of functions including serving as
prebiotics to stimulate the growth of beneficial intestinal bacteria, as receptor analogues to …

Chromatographic stationary phases based on porous graphitic carbon were invented 30
years ago, while columns have been commercially available for 20 years. This special …

The characterization of glycosylation is critical for obtaining a comprehensive view of the
regulation and functions of glycoproteins of interest. Due to the complex nature of …

A multitude of monoclonal IgG antibodies directed against a variety of therapeutic targets is
currently being developed and produced by biotechnological companies. The biological …

Abstract Two LC‐ESI‐MS methods for the analysis of antibody glycosylation are presented.
In the first approach, tryptic glycopeptides are separated by RP chromatography and …

Glycosylation defines the adhesive properties of animal cell surfaces and the surrounding
extracellular environments. Because cells respond to stimuli by altering glycan expression …

Porous graphitised carbon-liquid chromatography (PGC-LC) has been proven to be a
powerful technique for the analysis and characterisation of complex mixtures of isomeric and …