Exploring the use of animal models in craniofacial regenerative medicine: a narrative review

SA Mosaddad, A Hussain… - Tissue Engineering Part B …, 2024 - liebertpub.com
The craniofacial region contains skin, bones, cartilage, the temporomandibular joint (TMJ),
teeth, periodontal tissues, mucosa, salivary glands, muscles, nerves, and blood vessels …

Overlooked benefits of using polyclonal antibodies

CA Ascoli, B Aggeler - Biotechniques, 2018 - Taylor & Francis
The benefits of polyclonal antibodies as tools for assay-specific target discovery and
detection are numerous. As the future of basic research, diagnostics and biomarker …

RNA-Seq signatures normalized by mRNA abundance allow absolute deconvolution of human immune cell types

G Monaco, B Lee, W Xu, S Mustafah, YY Hwang… - Cell reports, 2019 - cell.com
The molecular characterization of immune subsets is important for designing effective
strategies to understand and treat diseases. We characterized 29 immune cell types within …

Immunofluorescence staining of paraffin sections step by step

S Zaqout, LL Becker, AM Kaindl - Frontiers in neuroanatomy, 2020 - frontiersin.org
Immunofluorescence staining is the most frequently applied technique to detect and
visualize various molecules in biological samples. Many protocols can be found in the …

Exosomes-mediated transfer of Itga2 promotes migration and invasion of prostate cancer cells by inducing epithelial-mesenchymal transition

R Gaballa, HEA Ali, MO Mahmoud, JS Rhim, HI Ali… - Cancers, 2020 - mdpi.com
Although integrin alpha 2 subunit (ITGA2) mediates cancer progression and metastasis, its
transfer by exosomes has not been investigated in prostate cancer (PCa). We aimed to …

Antibody validation for Western blot: By the user, for the user

L Pillai-Kastoori, S Heaton, SD Shiflett… - Journal of Biological …, 2020 - ASBMB
Well-characterized antibody reagents play a key role in the reproducibility of research
findings, and inconsistent antibody performance leads to variability in Western blotting and …

Distribution of phosphorylated alpha-synuclein in non-diseased brain implicates olfactory bulb mitral cells in synucleinopathy pathogenesis

BA Killinger, G Mercado, S Choi, T Tittle, Y Chu… - npj Parkinson's …, 2023 - nature.com
Synucleinopathies are neurodegenerative diseases characterized by pathological
inclusions called “Lewy pathology”(LP) that consist of aggregated alpha-synuclein …

[HTML][HTML] Nerve injury increases native CaV2. 2 trafficking in dorsal root ganglion mechanoreceptors

M Nieto-Rostro, R Patel, AH Dickenson, AC Dolphin - Pain, 2023 - journals.lww.com
Neuronal N-type (Ca V 2.2) voltage-gated calcium channels are essential for
neurotransmission from primary afferent terminals in the dorsal horn. In this study, we have …

Evaluation of commercially available glucagon receptor antibodies and glucagon receptor expression

AB Bomholt, CD Johansen, JB Christensen… - Communications …, 2022 - nature.com
Glucagon is a major regulator of metabolism and drugs targeting the glucagon receptor
(GCGR) are being developed. Insight into tissue and cell-specific expression of the GCGR is …

Antibody can get it right: confronting problems of antibody specificity and irreproducibility

A Schonbrunn - Molecular Endocrinology, 2014 - academic.oup.com
Antibodies are indispensable research reagents. Their use in endocrinology has exploded
since the original radioimmunoassays were developed to measure hormone levels. They …