Cancer metabolism is significantly altered from normal cellular metabolism allowing cancer
cells to adapt to changing microenvironments and maintain high rates of proliferation. In the …

The field of metabolic engineering is primarily concerned with improving the biological
production of value-added chemicals, fuels and pharmaceuticals through the design …

In metabolism, available free energy is limited and must be divided across pathway steps to
maintain a negative Δ G throughout. For each reaction, Δ G is log proportional both to a …

Over the past decade, a growing community of researchers has emerged around the use of
constraint-based reconstruction and analysis (COBRA) methods to simulate, analyze and …

Metabolites are the end products of cellular regulatory processes, and their levels can be
regarded as the ultimate response of biological systems to genetic or environmental …

Metabolic flux analysis using 13C-labeled substrates has become an important tool in
metabolic engineering. It allows the detailed quantification of all intracellular fluxes in the …

Stable isotope, and in particular 13C-based flux analysis, is the exclusive approach to
experimentally quantify the integrated responses of metabolic networks. Here we describe a …

Metabolic flux analysis (MFA) is one of the pillars of metabolic engineering. Over the past
three decades, it has been widely used to quantify intracellular metabolic fluxes in both …

Metabolic flux analysis (MFA) has emerged as a tool of great significance for metabolic
engineering and mammalian physiology. An important limitation of MFA, as carried out via …

Introduction Cells use multilayered regulatory systems to respond adequately to changing
environments or perturbations. Failure in regulation underlies cellular malfunctioning, loss of …