Optimal precision and accuracy in 4Pi-STORM using dynamic spline PSF models

M Bates, J Keller-Findeisen, A Przybylski, A Hüper… - Nature …, 2022 - nature.com
Coherent fluorescence imaging with two objective lenses (4Pi detection) enables single-
molecule localization microscopy with sub-10 nm spatial resolution in three dimensions …

Global fitting for high-accuracy multi-channel single-molecule localization

Y Li, W Shi, S Liu, I Cavka, YL Wu, U Matti… - Nature …, 2022 - nature.com
Multi-channel detection in single-molecule localization microscopy greatly increases
information content for various biological applications. Here, we present globLoc, a graphics …

[HTML][HTML] Review of 4Pi fluorescence nanoscopy

X Hao, Y Li, S Fu, Y Li, Y Xu, C Kuang, X Liu - Engineering, 2022 - Elsevier
Since the 1990s, continuous technical and scientific advances have defied the diffraction
limit in microscopy and enabled three-dimensional (3D) super-resolution imaging. An …

Implementation of a 4Pi-SMS super-resolution microscope

J Wang, ES Allgeyer, G Sirinakis, Y Zhang, K Hu… - Nature protocols, 2021 - nature.com
The development of single-molecule switching (SMS) fluorescence microscopy (also called
single-molecule localization microscopy) over the last decade has enabled researchers to …

Universal inverse modelling of point spread functions for SMLM localization and microscope characterization

S Liu, J Chen, J Hellgoth, LR Müller, B Ferdman… - Biophysical …, 2024 - cell.com
1617-Pos Contribution of desmoplakin isoforms to desmosome architecture Krishna A.
Patel, Collin M. Ainslie, Alexa L. Mattheyses. Department of Cell, Developmental and …

Optimal sampling rate for 3D single molecule localization

H Chang, S Fu, Y Li - Optics Express, 2023 - opg.optica.org
Resolution of single molecule localization microscopy (SMLM) depends on the localization
accuracy, which can be improved by utilizing engineered point spread functions (PSF) with …

Aberration correction for deformable-mirror-based remote focusing enables high-accuracy whole-cell super-resolution imaging

W Shi, Y He, J Wang, L Zhou, J Chen, L Zhou… - Photonics …, 2024 - opg.optica.org
Single-molecule localization microscopy (SMLM) enables three-dimensional (3D)
investigation of nanoscale structures in biological samples, offering unique insights into their …

Three-dimensional single particle tracking using 4π self-interference of temporally phase-shifted fluorescence

L Maurice, A Bilenca - Light: Science & Applications, 2023 - nature.com
Single particle tracking in three dimensions is an indispensable tool for studying dynamic
processes in various disciplines, including material sciences, physics, and biology, but often …

Fast and universal single molecule localization using multi-dimensional point spread functions

M Li, W Shi, S Liu, S Fu, Y Fei, L Zhou, Y Li - bioRxiv, 2023 - biorxiv.org
The recent development of single molecule imaging techniques has enabled not only high
accuracy spatial resolution imaging but also information rich functional imaging. Abundant …

[HTML][HTML] Interactive simulation and visualization of point spread functions in single molecule imaging

MC Schneider, F Hinterer, A Jesacher, GJ Schütz - Optics Communications, 2024 - Elsevier
The point spread function (PSF) is fundamental to any type of microscopy, most importantly
so for single-molecule localization techniques, where the exact PSF shape is crucial for …