The notochord is the defining structure of the chordates, and has essential roles in
vertebrate development. It serves as a source of midline signals that pattern surrounding …

The basic vertebrate body plan of the zebrafish embryo is established in the first 10 hours of
development. This period is characterized by the formation of the anterior-posterior and …

We describe a series of stages for development of the embryo of the zebrafish, Danio
(Brachydanio) rerio. We define seven broad periods of embryogenesis—the zygote …

The in situ hybridization (ISH) technique allows the sites of expression of particular genes to
be detected. This protocol describes ISH of digoxigenin-labeled antisense RNA probes to …

Costimulatory signals are required for activation of immune cells, but it is not known whether
they contribute to other biological systems. The development and homeostasis of the …

Mesoderm formation is critical for the establishment of the animal body plan and in
Drosophila requires the snail gene. This report concerns the cloning and expression pattern …

Identification and manipulation of the germ line are important to the study of model
organisms. Although zebrafish has recently emerged as a model for vertebrate …

Monocilia have been proposed to establish the left-right (LR) body axis in vertebrate
embryos by creating a directional fluid flow that triggers asymmetric gene expression. In …

A novel family of genes, characterized by the presence of a region of homology to the DNA‐
binding domain of the Brachyury (T) locus product, has recently been identified. The region …

The mouse T (Brachyury) gene is required for normal mesoderm development and the
extension of the body axis. Recently, two mutant alleles of a zebrafish gene, no tail (ntl) …