Genetic modification of clinical-grade T cells is undertaken to augment function, including
redirecting specificity for desired antigen. We and others have introduced a chimeric antigen …

Nonviral, DNA-mediated gene transfer is an alternative to viral delivery systems for
expressing new genes in cells and tissues. The Sleeping Beauty (SB) transposon system …

Efficient integration of functional genes is an essential prerequisite for successful gene
delivery such as cell transfection, animal transgenesis, and gene therapy. Gene delivery …

Two methods of systemic gene delivery have been extensively explored, using the mouse
as a model system: hydrodynamic delivery, wherein a DNA solution equivalent in volume to …

To develop T-cell therapies for B-cell malignancies we have developed a chimeric antigen
receptor (CAR) which when expressed on the cell surface redirects T-cell specificity for …