[HTML][HTML] The evolving role of investigative toxicology in the pharmaceutical industry

F Pognan, M Beilmann, H Boonen, A Czich… - Nature reviews drug …, 2023 - nature.com
For decades, preclinical toxicology was essentially a descriptive discipline in which
treatment-related effects were carefully reported and used as a basis to calculate safety …

Current challenges of iPSC-based disease modeling and therapeutic implications

MX Doss, A Sachinidis - Cells, 2019 - mdpi.com
Induced pluripotent stem cell (iPSC)-based disease modelling and the cell replacement
therapy approach have proven to be very powerful and instrumental in biomedical research …

An inflammatory aging clock (iAge) based on deep learning tracks multimorbidity, immunosenescence, frailty and cardiovascular aging

N Sayed, Y Huang, K Nguyen, Z Krejciova-Rajaniemi… - Nature aging, 2021 - nature.com
While many diseases of aging have been linked to the immunological system, immune
metrics capable of identifying the most at-risk individuals are lacking. From the blood …

Human cardiac organoids for the modelling of myocardial infarction and drug cardiotoxicity

DJ Richards, Y Li, CM Kerr, J Yao, GC Beeson… - Nature biomedical …, 2020 - nature.com
Environmental factors are the largest contributors to cardiovascular disease. Here we show
that cardiac organoids that incorporate an oxygen-diffusion gradient and that are stimulated …

Induced pluripotent stem cell technology: a decade of progress

Y Shi, H Inoue, JC Wu, S Yamanaka - Nature reviews Drug discovery, 2017 - nature.com
Since the advent of induced pluripotent stem cell (iPSC) technology a decade ago,
enormous progress has been made in stem cell biology and regenerative medicine. Human …

Single-cell RNA sequencing in cardiovascular development, disease and medicine

DT Paik, S Cho, L Tian, HY Chang… - Nature Reviews Cardiology, 2020 - nature.com
Advances in single-cell RNA sequencing (scRNA-seq) technologies in the past 10 years
have had a transformative effect on biomedical research, enabling the profiling and analysis …

Addressing variability in iPSC-derived models of human disease: guidelines to promote reproducibility

V Volpato, C Webber - Disease models & mechanisms, 2020 - journals.biologists.com
Induced pluripotent stem cell (iPSC) technologies have provided in vitro models of
inaccessible human cell types, yielding new insights into disease mechanisms especially for …

Wnt activation and reduced cell-cell contact synergistically induce massive expansion of functional human iPSC-derived cardiomyocytes

JW Buikema, S Lee, WR Goodyer, RG Maas… - Cell stem cell, 2020 - cell.com
Modulating signaling pathways including Wnt and Hippo can induce cardiomyocyte
proliferation in vivo. Applying these signaling modulators to human induced pluripotent stem …

Metabolically driven maturation of human-induced-pluripotent-stem-cell-derived cardiac microtissues on microfluidic chips

N Huebsch, B Charrez, G Neiman, B Siemons… - Nature biomedical …, 2022 - nature.com
The immature physiology of cardiomyocytes derived from human induced pluripotent stem
cells (hiPSCs) limits their utility for drug screening and disease modelling. Here we show …

Engineering cardiac muscle tissue: a maturating field of research

F Weinberger, I Mannhardt, T Eschenhagen - Circulation research, 2017 - Am Heart Assoc
Twenty years after the initial description of a tissue engineered construct, 3-dimensional
human cardiac tissues of different kinds are now generated routinely in many laboratories …