Microbial cell factories (MCFs) are of considerable interest to convert low value renewable
substrates to biofuels and high value chemicals. This review highlights the progress of …

Glycans and glycosylated biomolecules are directly involved in almost every biological
process as well as the etiology of most major diseases. Hence, glycoscience knowledge is …

The Campylobacter jejuni protein glycosylation locus (pgl) encodes machinery for
asparagine-linked (N-linked) glycosylation and serves as the archetype for bacterial N …

Background Glycoconjugated vaccines composed of polysaccharide antigens covalently
linked to immunogenic carrier proteins have proved to belong to the most effective and …

Background Bacterial surface glycans are assembled by glycosyltransferases (GTs) that
transfer sugar monomers to long-chained lipid carriers. Most bacteria employ the 55-carbon …

Background Glycoengineering, in the biotechnology workhorse bacterium, Escherichia coli,
is a rapidly evolving field, particularly for the production of glycoconjugate vaccine …

The recent advancement in the human glycome and progress in the development of an
inclusive network of glycosylation pathways allow the incorporation of suitable machinery for …

Site selectivity of protein N-linked glycosylation is dependent on many factors, including
accessibility of the modification site, amino acid composition of the glycosylation consensus …

Protein glycosylation, which involves the addition of carbohydrate chains to amino acid side
chains, imparts essential properties to proteins, offering immense potential in synthetic …

Highlights•Glycosylation of therapeutic proteins in E. coli.•Increasing the efficiency of
glycosylation.•Producing the eukaryotic glycan within E. coli.•Targeting glycans towards a …