Cell wall construction in Saccharomyces cerevisiae

FM Klis, A Boorsma, PWJ De Groot - Yeast, 2006 - Wiley Online Library
In this review, we discuss new insights in cell wall architecture and cell wall construction in
the ascomycetous yeast Saccharomyces cerevisiae. Transcriptional profiling studies …

Features and functions of covalently linked proteins in fungal cell walls

PWJ De Groot, AF Ram, FM Klis - Fungal genetics and biology, 2005 - Elsevier
The cell walls of many ascomycetous yeasts consist of an internal network of stress-bearing
polysaccharides, which serve as a scaffold for a dense external layer of glycoproteins. GPI …

Peeling the onion: the outer layers of Cryptococcus neoformans

DP Agustinho, LC Miller, LX Li… - Memórias do Instituto …, 2018 - SciELO Brasil
Cryptococcus neoformans is an opportunistic fungal pathogen that is ubiquitous in the
environment. It causes a deadly meningitis that is responsible for over 180,000 deaths …

Evolutionary overview of molecular interactions and enzymatic activities in the yeast cell walls

R Teparić, M Lozančić, V Mrša - International journal of molecular …, 2020 - mdpi.com
Fungal cell walls are composed of a polysaccharide network that serves as a scaffold in
which different glycoproteins are embedded. Investigation of fungal cell walls, besides …

Functional analysis of the cysteine residues and the repetitive sequence of Saccharomyces cerevisiae Pir4/Cis3: the repetitive sequence is needed for binding to the …

L Castillo, AI Martinez, A Garcerá, M Victoria Elorza… - Yeast, 2003 - Wiley Online Library
Identification of PIR/CIS3 gene was carried out by amino‐terminal sequencing of a protein
band released by β‐mercaptoethanol (β‐ME) from S. cerevisiae mnn9 cell walls. The protein …

Yeast cell surface display for lipase whole cell catalyst and its applications

Y Liu, R Zhang, Z Lian, S Wang, AT Wright - Journal of Molecular Catalysis …, 2014 - Elsevier
The cell surface display technique allows for the expression of target proteins or peptides on
the microbial cell surface by fusing an appropriate protein as an anchoring motif. Yeast …

Role of Pir1 in the construction of the Candida albicans cell wall

AI Martínez, L Castillo, A Garcera, MV Elorza… - …, 2004 - microbiologyresearch.org
Searches in a Candida albicans database (http://genolist. pasteur. fr/CandidaDB/) identified
two Individual Protein Files (IPF 15363 and 19968) whose deduced amino acid sequences …

A molecular and genomic view of the fungal cell wall

FM Klis, AFJ Ram, PWJ De Groot - Biology of the fungal cell, 2007 - Springer
The fungal cell wall is anessential organelle that accounts for 15–30% of the cellular dry
weight and therefore represents a substantial metabolic investment for the cell. In return …

Cell Surface Display of Yarrowia lipolytica Lipase Lip2p Using the Cell Wall Protein YlPir1p, Its Characterization, and Application as a Whole-Cell Biocatalyst

EY Yuzbasheva, TV Yuzbashev… - Applied Biochemistry …, 2015 - Springer
Abstract The Yarrowia lipolytica lipase Lip2p was displayed on the yeast cell surface via N-
terminal fusion variant using cell wall protein YlPir1p. The hydrolytic activity of the lipase …

The contribution of Pir protein family to yeast cell surface display

N Yang, Z Yu, D Jia, Z Xie, K Zhang, Z Xia, L Lei… - Applied microbiology …, 2014 - Springer
Proteins with internal repeats (Pir) in the Baker's yeast are located on the cell wall and
include four highly homologous members. Recently, Pir proteins have become increasingly …