Directed evolution has matured to a routinely applied algorithm to tailor enzyme properties
to meet the demands in various applications. In order to free directed enzyme evolution from …

Genetic diversity creation is a core technology in directed evolution where a high quality
mutant library is crucial to its success. Owing to its importance, the technology in genetic …

White biotechnology, or industrial biotechnology, is drawing much attention as a solution to
produce energy, chemicals and other materials from renewable resources. White …

Enzyme immobilization is extensively studied to improve enzyme properties in catalysis and
analytical applications. Here, we introduce a simple and versatile enzyme immobilization …

Protein engineering is widely used to generate proteins with novel or enhanced function.
However, manipulating protein function in the laboratory can prove laborious, protracted and …

Protein re‐engineering by directed evolution has become a standard approach for tailoring
enzymes in many fields of science and industry. Advances in screening formats and …

Concerns of phosphorus pollution and its impact on environments have driven the
biotechnological development of phytases. Phosphoric acid, inositol phosphate, or inositols …

During the last decade, directed evolution has become a standard protein engineering
strategy to reengineer proteins for industrial applications under high stress conditions (eg …

Enzyme immobilization has been widely used to improve the stability and recyclability of
enzymes in industrial processes. In this work, a sortase-mediated and therefore selective …

A new cellulolytic strain of Chryseobacterium genus was screened from the dung of a cattle
fed with cereal straw. A putative cellulase gene (cb GH 5) belonging to glycoside hydrolase …