The chitin-binding domain of a GH-18 chitinase from Vibrio harveyi is crucial for chitin-chitinase interactions

W Suginta, P Sirimontree, N Sritho, T Ohnuma… - International journal of …, 2016 - Elsevier
Vibrio harveyi chitinase A (Vh ChiA) is a GH-18 glycosyl hydrolase with a structure
containing three distinct domains: i) the N-terminal chitin-binding domain; ii) the (α/β) 8 TIM …

Reversibility and two state behaviour in the thermal unfolding of oligomeric TIM barrel proteins

S Romero-Romero, M Costas… - Physical Chemistry …, 2015 - pubs.rsc.org
Temperature is one of the main variables that modulate protein function and stability.
Thermodynamic studies of oligomeric proteins, the dominant protein natural form, have been …

The Crystal Structure of a Streptomyces thermoviolaceus Thermophilic Chitinase Known for Its Refolding Efficiency

PH Malecki, M Bejger, W Rypniewski… - International Journal of …, 2020 - mdpi.com
Analyzing the structure of proteins from extremophiles is a promising way to study the rules
governing the protein structure, because such proteins are results of structural and …

Stability, Unfolding, and Structural Changes of Cofactor-Free 1H-3-Hydroxy-4-oxoquinaldine 2,4-Dioxygenase

B Beermann, J Guddorf, K Boehm, A Albers… - Biochemistry, 2007 - ACS Publications
Stability, unfolding mechanism, spectroscopic, densimetric, and structural characteristics of
the oxidatively stable C69S variant (HodC) of 1 H-3-hydroxy-4-oxoquinaldine 2, 4 …

The differential stability of the left and right domains of papain

HA Sathish, PR Kumar, V Prakash - Process biochemistry, 2009 - Elsevier
We have investigated the differential stability of the two domains of papain, a broad specific
cysteine protease, which is one of the most commonly used enzyme in various industries …

Qualification of biophysical methods for the analysis of protein therapeutics

Y Jiang, C Li, J Gabrielson - Biophysics for Therapeutic Protein …, 2013 - Springer
Biophysical techniques such as analytical ultracentrifugation (AUC), circular dichroism (CD),
and differential scanning calorimetry (DSC) have been widely used by the …

[HTML][HTML] The stability of the TIM-barrel domain of a psychrophilic chitinase

P Stavros, PH Malecki, M Theodoridou… - … and Biophysics Reports, 2015 - Elsevier
Chitinase 60 from the psychrophilic bacterium Moritella marina (MmChi60) is a four-domain
protein whose structure revealed flexible hinge regions between the domains, yielding …

[PDF][PDF] Structural studies of chitinolytic enzymes fromPyrococcus chitonophagus

K Biniek-Antosiak - portal.ichb.pl
Extremophile is an organism that is adapted to or requires extreme ranges of environmental
factors to live. In most cases, extremophiles are single-celled organisms that are mainly …

[PDF][PDF] Survival in extremes

PH Malecki, W Rypniewski - BioTechnologia. Journal of …, 2013 - bibliotekanauki.pl
Demand is growing for extremozymes for biotechnological purposes. Extremophilic
organisms are a natural source of enzymes that possess features desired by the …

Mecanismos de dissociação das subunidades alfa e Beta da Na, K-ATPase por agentes químicos e físicos: comparação entre a enzima solubilizada e reconstituída …

CF Rigos - 2007 - teses.usp.br
A Na, K-ATPase é uma proteína encontrada na membrana plasmática de praticamente
todas as células animais, que utiliza a energia derivada da hidrólise do ATP para …