Applications of X-ray powder diffraction in protein crystallography and drug screening

M Spiliopoulou, A Valmas, DP Triandafillidis… - Crystals, 2020 - mdpi.com
Providing fundamental information on intra/intermolecular interactions and physicochemical
properties, the three-dimensional structural characterization of biological macromolecules is …

Enhancement of Thermostability of Aspergillus flavus Urate Oxidase by Immobilization on the Ni-Based Magnetic Metal–Organic Framework

N Motamedi, M Barani, A Lohrasbi-Nejad, M Mortazavi… - Nanomaterials, 2021 - mdpi.com
The improvement in the enzyme activity of Aspergillus flavus urate oxidase (Uox) was
attained by immobilizing it on the surface of a Ni-based magnetic metal–organic framework …

In quest for improved drugs against diabetes: The added value of X-ray powder diffraction methods

F Karavassili, A Valmas, S Fili, CD Georgiou… - Biomolecules, 2017 - mdpi.com
Human insulin (HI) is a well-characterized natural hormone which regulates glycose levels
into the blood-stream and is widely used for diabetes treatment. Numerous studies have …

Cloning and expression of Aspergillus flavus urate oxidase in Pichia pastoris

R Fazel, N Zarei, N Ghaemi, MM Namvaran, S Enayati… - Springerplus, 2014 - Springer
Urate oxidase is an important enzyme with therapeutic and diagnostic applications.
Rasburicase is a recombinant urate oxidase enzyme approved by FDA to use in the …

New perspectives in macromolecular powder diffraction using single-photon-counting strip detectors: high-resolution structure of the pharmaceutical peptide …

M Spiliopoulou, F Karavassili… - … A: Foundations and …, 2021 - journals.iucr.org
Advances in instrumentation, as well as the development of powerful crystallographic
software have significantly facilitated the collection of high-resolution diffraction data and …

Rietveld refinement for macromolecular powder diffraction

M Spiliopoulou, DP Triandafillidis, A Valmas… - Crystal Growth & …, 2020 - ACS Publications
Powder X-ray diffraction (PXRD) has been employed extensively for the structural
characterization of materials, quantitative analysis of multicomponent mixtures, phase …

[PDF][PDF] Human insulin polymorphism upon ligand binding and pH variation: the case of 4-ethylresorcinol

S Fili, A Valmas, M Norrman, G Schluckebier… - IUCrJ, 2015 - journals.iucr.org
This study focuses on the effects of the organic ligand 4-ethylresorcinol on the crystal
structure of human insulin using powder X-ray crystallography. For this purpose, systematic …

Structural studies of human insulin cocrystallized with phenol or resorcinol via powder diffraction

F Karavassili, AE Giannopoulou, E Kotsiliti… - … Section D: Biological …, 2012 - journals.iucr.org
The effects of the ligands phenol and resorcinol on the crystallization of human insulin have
been investigated as a function of pH. Powder diffraction data were used to characterize …

High-resolution powder X-ray data reveal the T6 hexameric form of bovine insulin

I Margiolaki, AE Giannopoulou, JP Wright… - … Section D: Biological …, 2013 - journals.iucr.org
A series of bovine insulin samples were obtained as 14 polycrystalline precipitates at room
temperature in the pH range 5.0–7.6. High-resolution powder X-ray diffraction data were …

Novel crystalline phase and first-order phase transitions of human insulin complexed with two distinct phenol derivatives

A Valmas, K Magiouf, S Fili, M Norrman… - … Section D: Biological …, 2015 - journals.iucr.org
The primary focus of the present work is the study of the effects that two ligands and the
crystallization pH have on the crystalline forms of human insulin. For this purpose, human …