Recent advances in systems and synthetic biology approaches for developing novel cell-factories in non-conventional yeasts

P Patra, M Das, P Kundu, A Ghosh - Biotechnology Advances, 2021 - Elsevier
Microbial bioproduction of chemicals, proteins, and primary metabolites from cheap carbon
sources is currently an advancing area in industrial research. The model yeast …

Engineering strategies for enhanced production of protein and bio-products in Pichia pastoris: a review

Z Yang, Z Zhang - Biotechnology advances, 2018 - Elsevier
Pichia pastoris has been recognized as one of the most industrially important hosts for
heterologous protein production. Despite its high protein productivity, the optimization of P …

Methanol biotransformation toward high-level production of fatty acid derivatives by engineering the industrial yeast Pichia pastoris

P Cai, X Wu, J Deng, L Gao, Y Shen… - Proceedings of the …, 2022 - National Acad Sciences
Methanol-based biorefinery is a promising strategy to achieve carbon neutrality goals by
linking CO2 capture and solar energy storage. As a typical methylotroph, Pichia pastoris …

Systems metabolic engineering strategies: integrating systems and synthetic biology with metabolic engineering

KR Choi, WD Jang, D Yang, JS Cho, D Park… - Trends in …, 2019 - cell.com
Metabolic engineering allows development of microbial strains efficiently producing
chemicals and materials, but it requires much time, effort, and cost to make the strains …

Biocatalyst for the synthesis of natural flavouring compounds as food additives: Bridging the gap for a more sustainable industrial future

S Singh, PK Sharma, S Chaturvedi, P Kumar… - Food Chemistry, 2024 - Elsevier
Biocatalysis entails the use of purified enzymes in the manufacturing of flavouring chemicals
food industry as well as at the laboratory level. These biocatalysts can significantly …

[HTML][HTML] Metabolic engineering of Pichia pastoris

DA Peña, B Gasser, J Zanghellini, MG Steiger… - Metabolic …, 2018 - Elsevier
Besides its use for efficient production of recombinant proteins the methylotrophic yeast
Pichia pastoris (syn. Komagataella spp.) has been increasingly employed as a platform to …

Industrial Production of Proteins with Pichia pastorisKomagataella phaffii

GD Barone, A Emmerstorfer-Augustin, A Biundo… - Biomolecules, 2023 - mdpi.com
Since the mid-1960s, methylotrophic yeast Komagataella phaffii (previously described as
Pichia pastoris) has received increasing scientific attention. The interest for the industrial …

Heterologous Protein Expression in Pichia pastoris: Latest Research Progress and Applications

V Juturu, JC Wu - ChemBioChem, 2018 - Wiley Online Library
Pichia pastoris is a well‐known platform strain for heterologous protein expression. Over the
past five years, different strategies to improve the efficiency of recombinant protein …

Biosynthesis of catharanthine in engineered Pichia pastoris

J Gao, Y Zuo, F Xiao, Y Wang, D Li, J Xu, C Ye… - Nature …, 2023 - nature.com
Catharanthine can be coupled with vindoline to synthesize vinblastine and vincristine, which
have been used clinically as potent anticancer drugs. However, the structural complexity …

Barriers and opportunities in bio-based production of hydrocarbons

YJ Zhou, EJ Kerkhoven, J Nielsen - Nature Energy, 2018 - nature.com
Global climate change caused by the accumulation of greenhouse gases (GHGs) has
caused concerns regarding the continued reliance on fossil fuels as our primary energy …