Cell proliferation and differentiation show a remarkable inverse relationship. Precursor cells
continue division before acquiring a fully differentiated state, while terminal differentiation …

Establishing and maintaining cell identity depends on the proper regulation of gene
expression, as specified by transcription factors and reinforced by epigenetic mechanisms …

Nucleosomes help structure chromosomes by compacting DNA into fibers. To gain insight
into how nucleosomes are arranged in vivo, we combined quantitative super-resolution …

Total RNA sequencing has been used to reveal poly (A) and non-poly (A) RNA expression,
RNA processing and enhancer activity. To date, no method for full-length total RNA …

Much of the mammalian genome is transcribed, generating long non-coding RNAs
(lncRNAs) that can undergo post-transcriptional surveillance whereby only a subset of the …

Cellular differentiation is, by definition, epigenetic. Genome-wide profiling of pluripotent cells
and differentiated cells suggests global chromatin remodelling during differentiation, which …

Next-generation sequencing allows the analysis of genomes, including those representing
disease states. However, the causes of most disorders are multifactorial, and systems-level …

While the long noncoding RNAs (ncRNAs) constitute a large portion of the mammalian
transcriptome, their biological functions has remained elusive. A few long ncRNAs that have …

Pluripotency is the remarkable capacity of a single cell to engender all the specialized cell
types of an adult organism. This property can be captured indefinitely through derivation of …

Mouse embryonic stem (ES) cells grown in serum exhibit greater heterogeneity in
morphology and expression of pluripotency factors than ES cells cultured in defined medium …