Considerations and best practices in animal science 16S ribosomal RNA gene sequencing microbiome studies
Microbiome studies in animal science using 16S rRNA gene sequencing have become
increasingly common in recent years as sequencing costs continue to fall and bioinformatic …
increasingly common in recent years as sequencing costs continue to fall and bioinformatic …
[HTML][HTML] Genetic detection of freshwater harmful algal blooms: A review focused on the use of environmental DNA (eDNA) in Microcystis aeruginosa and Prymnesium …
SM Feist, RF Lance - Harmful Algae, 2021 - Elsevier
Recurrence and severity of harmful algal blooms (HABs) are increasing due to a number of
factors, including human practices and climate change. Sensitive and robust methods that …
factors, including human practices and climate change. Sensitive and robust methods that …
Multiplex detection of foodborne pathogens by real-time loop-mediated isothermal amplification on a digital microfluidic chip
M Xie, T Chen, X Xin, Z Cai, C Dong, B Lei - Food control, 2022 - Elsevier
Rapid detection of foodborne pathogens is critical to assure food quality. In this study, a
digital microfluidic (DMF) chip in combination with loop-mediated isothermal amplification …
digital microfluidic (DMF) chip in combination with loop-mediated isothermal amplification …
[HTML][HTML] Phospholipid fatty acid (PLFA) analysis as a tool to estimate absolute abundances from compositional 16S rRNA bacterial metabarcoding data
Microbial biodiversity monitoring through the analysis of DNA extracted from environmental
samples is increasingly popular because it is perceived as being rapid, cost-effective, and …
samples is increasingly popular because it is perceived as being rapid, cost-effective, and …
[HTML][HTML] Gut probiotic Lactobacillus rhamnosus attenuates PDE4B-mediated interleukin-6 induced by SARS-CoV-2 membrane glycoprotein
MT Pham, AJ Yang, MS Kao, U Gankhuyag… - The Journal of …, 2021 - Elsevier
Membrane glycoprotein is the most abundant protein of severe acute respiratory syndrome
coronavirus 2 (SARS-CoV-2), but its role in coronavirus disease 2019 (COVID-19) has not …
coronavirus 2 (SARS-CoV-2), but its role in coronavirus disease 2019 (COVID-19) has not …
The current status and trends of DNA extraction
X Ye, B Lei - Bioessays, 2023 - Wiley Online Library
DNA extraction, playing an irreplaceable role in molecular biology as it is an essential step
prior to various downstream biological analyses. Thus, the accuracy and reliability of …
prior to various downstream biological analyses. Thus, the accuracy and reliability of …
A simple, inexpensive alkaline method for bacterial DNA extraction from environmental samples for PCR surveillance and microbiome analyses
DNA extraction for downstream molecular diagnostic applications can be an expensive, time-
consuming process. We devised a method to quickly extract total bacterial DNA from …
consuming process. We devised a method to quickly extract total bacterial DNA from …
Highly efficient on-chip photothermal cell lysis for nucleic acid extraction using localized plasmonic heating of strongly absorbing Au nanoislands
Cell lysis serves as an essential role in the sample preparation for intracellular material
extraction in lab-on-a-chip applications. However, recent microfluidic-based cell lysis chips …
extraction in lab-on-a-chip applications. However, recent microfluidic-based cell lysis chips …
Novel Carbapenem-Resistant Klebsiella pneumoniae ST147 Coharboring blaNDM-1, blaOXA-48 and Extended-Spectrum β-Lactamases from Pakistan
Purpose The emergence of multidrug-resistant Klebsiella pneumoniae (K. pneumoniae) is
associated with the acquisition of multiple carbapenemases. Their clonal spread is a …
associated with the acquisition of multiple carbapenemases. Their clonal spread is a …
Simultaneous microbial capture and nucleic acid extraction from wastewater with minimal pre-processing and high recovery efficiency
The COVID-19 pandemic accelerated research towards developing low-cost assays for
automated urban wastewater monitoring assay that can be integrated into an environmental …
automated urban wastewater monitoring assay that can be integrated into an environmental …