Considerations and best practices in animal science 16S ribosomal RNA gene sequencing microbiome studies

MD Weinroth, AD Belk, C Dean, N Noyes… - Journal of animal …, 2022 - academic.oup.com
Microbiome studies in animal science using 16S rRNA gene sequencing have become
increasingly common in recent years as sequencing costs continue to fall and bioinformatic …

[HTML][HTML] Genetic detection of freshwater harmful algal blooms: A review focused on the use of environmental DNA (eDNA) in Microcystis aeruginosa and Prymnesium …

SM Feist, RF Lance - Harmful Algae, 2021 - Elsevier
Recurrence and severity of harmful algal blooms (HABs) are increasing due to a number of
factors, including human practices and climate change. Sensitive and robust methods that …

Multiplex detection of foodborne pathogens by real-time loop-mediated isothermal amplification on a digital microfluidic chip

M Xie, T Chen, X Xin, Z Cai, C Dong, B Lei - Food control, 2022 - Elsevier
Rapid detection of foodborne pathogens is critical to assure food quality. In this study, a
digital microfluidic (DMF) chip in combination with loop-mediated isothermal amplification …

[HTML][HTML] Phospholipid fatty acid (PLFA) analysis as a tool to estimate absolute abundances from compositional 16S rRNA bacterial metabarcoding data

N Lewe, S Hermans, G Lear, LT Kelly… - Journal of …, 2021 - Elsevier
Microbial biodiversity monitoring through the analysis of DNA extracted from environmental
samples is increasingly popular because it is perceived as being rapid, cost-effective, and …

[HTML][HTML] Gut probiotic Lactobacillus rhamnosus attenuates PDE4B-mediated interleukin-6 induced by SARS-CoV-2 membrane glycoprotein

MT Pham, AJ Yang, MS Kao, U Gankhuyag… - The Journal of …, 2021 - Elsevier
Membrane glycoprotein is the most abundant protein of severe acute respiratory syndrome
coronavirus 2 (SARS-CoV-2), but its role in coronavirus disease 2019 (COVID-19) has not …

The current status and trends of DNA extraction

X Ye, B Lei - Bioessays, 2023 - Wiley Online Library
DNA extraction, playing an irreplaceable role in molecular biology as it is an essential step
prior to various downstream biological analyses. Thus, the accuracy and reliability of …

A simple, inexpensive alkaline method for bacterial DNA extraction from environmental samples for PCR surveillance and microbiome analyses

A Shwani, B Zuo, A Alrubaye, J Zhao, DD Rhoads - Applied Sciences, 2023 - mdpi.com
DNA extraction for downstream molecular diagnostic applications can be an expensive, time-
consuming process. We devised a method to quickly extract total bacterial DNA from …

Highly efficient on-chip photothermal cell lysis for nucleic acid extraction using localized plasmonic heating of strongly absorbing Au nanoislands

ES Yu, BH Kang, MS Ahn, JH Jung… - … Applied Materials & …, 2023 - ACS Publications
Cell lysis serves as an essential role in the sample preparation for intracellular material
extraction in lab-on-a-chip applications. However, recent microfluidic-based cell lysis chips …

Novel Carbapenem-Resistant Klebsiella pneumoniae ST147 Coharboring blaNDM-1, blaOXA-48 and Extended-Spectrum β-Lactamases from Pakistan

AJ Gondal, S Saleem, S Jahan… - Infection and Drug …, 2020 - Taylor & Francis
Purpose The emergence of multidrug-resistant Klebsiella pneumoniae (K. pneumoniae) is
associated with the acquisition of multiple carbapenemases. Their clonal spread is a …

Simultaneous microbial capture and nucleic acid extraction from wastewater with minimal pre-processing and high recovery efficiency

S Ahuja, S Tallur, K Kondabagil - Science of The Total Environment, 2024 - Elsevier
The COVID-19 pandemic accelerated research towards developing low-cost assays for
automated urban wastewater monitoring assay that can be integrated into an environmental …