F-actin cytoskeleton network self-organization through competition and cooperation

RS Kadzik, KE Homa, DR Kovar - Annual review of cell and …, 2020 - annualreviews.org
Many fundamental cellular processes such as division, polarization, endocytosis, and
motility require the assembly, maintenance, and disassembly of filamentous actin (F-actin) …

Interactions of SARS-CoV-2 envelope protein with amilorides correlate with antiviral activity

SH Park, H Siddiqi, DV Castro, AA De Angelis… - PLoS …, 2021 - journals.plos.org
SARS-CoV-2 is the novel coronavirus that is the causative agent of COVID-19, a sometimes-
lethal respiratory infection responsible for a world-wide pandemic. The envelope (E) protein …

Calponin-homology domain mediated bending of membrane-associated actin filaments

S Palani, S Ghosh, E Ivorra-Molla, S Clarke… - Elife, 2021 - elifesciences.org
Actin filaments are central to numerous biological processes in all domains of life. Driven by
the interplay with molecular motors, actin binding and actin modulating proteins, the actin …

DYRK kinase Pom1 drives F-BAR protein Cdc15 from the membrane to promote medial division

R Bhattacharjee, MSC Mangione, M Wos… - Molecular biology of …, 2020 - Am Soc Cell Biol
In many organisms, positive and negative signals cooperate to position the division site for
cytokinesis. In the rod-shaped fission yeast Schizosaccharomyces pombe, symmetric …

mNG-tagged fusion proteins and nanobodies to visualize tropomyosins in yeast and mammalian cells

T Hatano, TC Lim, I Billault-Chaumartin… - Journal of cell …, 2022 - journals.biologists.com
Tropomyosins are structurally conserved α-helical coiled-coil proteins that bind along the
length of filamentous actin (F-actin) in fungi and animals. Tropomyosins play essential roles …

Visualizing the in vitro assembly of tropomyosin/actin filaments using TIRF microscopy

M Janco, I Dedova, NS Bryce, EC Hardeman… - Biophysical …, 2020 - Springer
Tropomyosins are elongated alpha-helical proteins that form co-polymers with most actin
filaments within a cell and play important roles in the structural and functional diversification …

Novel human cell expression method reveals the role and prevalence of posttranslational modification in nonmuscle tropomyosins

PJ Carman, KR Barrie, R Dominguez - Journal of Biological Chemistry, 2021 - ASBMB
Biochemical studies require large quantities of proteins, which are typically obtained using
bacterial overexpression. However, the folding machinery in bacteria is inadequate for …

Dynamics of Tpm1. 8 domains on actin filaments with single-molecule resolution

I Bareja, H Wioland, M Janco, PR Nicovich… - Molecular Biology of …, 2020 - Am Soc Cell Biol
Tropomyosins regulate the dynamics and functions of the actin cytoskeleton by forming long
chains along the two strands of actin filaments that act as gatekeepers for the binding of …

mNeonGreen-tagged fusion proteins and nanobodies reveal localization of tropomyosin to patches, cables, and contractile actomyosin rings in live yeast cells

T Hatano, TC Lim, I Billault-Chaumartin, A Dhar, Y Gu… - bioRxiv, 2022 - biorxiv.org
Tropomyosins are structurally conserved α-helical coiled-coil dimeric proteins that bind
along the length of filamentous actin (F-actin) in fungi and animals. Tropomyosins play …

Acetylation of fission yeast tropomyosin does not promote differential association with cognate formins

Q Tang, LW Pollard, KE Homa, DR Kovar… - …, 2023 - Wiley Online Library
It was proposed from cellular studies that S. pombe tropomyosin Cdc8 (Tpm) segregates into
two populations due to the presence or absence of an amino‐terminal acetylation that …