Droplet-based microfluidic systems generate, manipulate and control sub-microlitre droplets
enclosed within an immiscible carrier fluid. Owing to a number of remarkable features, such …

This perspective considers ways in which the field of microfluidics can increase its impact by
improving existing technologies and enabling new functionalities. We highlight applications …

Resolving the spatial distribution of RNA and protein in tissues at subcellular resolution is a
challenge in the field of spatial biology. We describe spatial molecular imaging, a system …

Embryonic stem (ES) cells can undergo many aspects of mammalian embryogenesis in
vitro,,,–, but their developmental potential is substantially extended by interactions with …

Neutrophils accumulate in solid tumors, and their abundance correlates with poor prognosis.
Neutrophils are not homogeneous, however, and could play different roles in cancer …

Cytotoxic T lymphocyte (CTL) responses against tumors are maintained by stem-like
memory cells that self-renew but also give rise to effector-like cells. The latter gradually lose …

Most methods for single-cell transcriptome sequencing amplify the termini of polyadenylated
transcripts, capturing only a small fraction of the total cellular transcriptome. This precludes …

Brain organoids have been used to recapitulate the processes of brain development and
related diseases. However, the lack of vasculatures, which regulate neurogenesis and brain …

The recent boom in microfluidics and combinatorial indexing strategies, combined with low
sequencing costs, has empowered single-cell sequencing technology. Thousands—or even …

Glioblastoma (GBM) is an incurable primary malignant brain cancer hallmarked with a
substantial protumorigenic immune component. Knowledge of the GBM immune …