Droplet-based microfluidics
T Moragues, D Arguijo, T Beneyton, C Modavi… - Nature Reviews …, 2023 - nature.com
Droplet-based microfluidic systems generate, manipulate and control sub-microlitre droplets
enclosed within an immiscible carrier fluid. Owing to a number of remarkable features, such …
enclosed within an immiscible carrier fluid. Owing to a number of remarkable features, such …
An outlook on microfluidics: the promise and the challenge
S Battat, DA Weitz, GM Whitesides - Lab on a Chip, 2022 - pubs.rsc.org
This perspective considers ways in which the field of microfluidics can increase its impact by
improving existing technologies and enabling new functionalities. We highlight applications …
improving existing technologies and enabling new functionalities. We highlight applications …
High-plex imaging of RNA and proteins at subcellular resolution in fixed tissue by spatial molecular imaging
S He, R Bhatt, C Brown, EA Brown, DL Buhr… - Nature …, 2022 - nature.com
Resolving the spatial distribution of RNA and protein in tissues at subcellular resolution is a
challenge in the field of spatial biology. We describe spatial molecular imaging, a system …
challenge in the field of spatial biology. We describe spatial molecular imaging, a system …
[HTML][HTML] Embryo model completes gastrulation to neurulation and organogenesis
Embryonic stem (ES) cells can undergo many aspects of mammalian embryogenesis in
vitro,,,–, but their developmental potential is substantially extended by interactions with …
vitro,,,–, but their developmental potential is substantially extended by interactions with …
A neutrophil response linked to tumor control in immunotherapy
Neutrophils accumulate in solid tumors, and their abundance correlates with poor prognosis.
Neutrophils are not homogeneous, however, and could play different roles in cancer …
Neutrophils are not homogeneous, however, and could play different roles in cancer …
[HTML][HTML] CXCR6 positions cytotoxic T cells to receive critical survival signals in the tumor microenvironment
M Di Pilato, R Kfuri-Rubens, JN Pruessmann, AJ Ozga… - Cell, 2021 - cell.com
Cytotoxic T lymphocyte (CTL) responses against tumors are maintained by stem-like
memory cells that self-renew but also give rise to effector-like cells. The latter gradually lose …
memory cells that self-renew but also give rise to effector-like cells. The latter gradually lose …
[HTML][HTML] High-throughput total RNA sequencing in single cells using VASA-seq
Most methods for single-cell transcriptome sequencing amplify the termini of polyadenylated
transcripts, capturing only a small fraction of the total cellular transcriptome. This precludes …
transcripts, capturing only a small fraction of the total cellular transcriptome. This precludes …
Generation of vascularized brain organoids to study neurovascular interactions
XY Sun, XC Ju, Y Li, PM Zeng, J Wu, YY Zhou… - elife, 2022 - elifesciences.org
Brain organoids have been used to recapitulate the processes of brain development and
related diseases. However, the lack of vasculatures, which regulate neurogenesis and brain …
related diseases. However, the lack of vasculatures, which regulate neurogenesis and brain …
[HTML][HTML] Eleven grand challenges in single-cell data science
The recent boom in microfluidics and combinatorial indexing strategies, combined with low
sequencing costs, has empowered single-cell sequencing technology. Thousands—or even …
sequencing costs, has empowered single-cell sequencing technology. Thousands—or even …
[HTML][HTML] Single-cell RNA sequencing reveals evolution of immune landscape during glioblastoma progression
Glioblastoma (GBM) is an incurable primary malignant brain cancer hallmarked with a
substantial protumorigenic immune component. Knowledge of the GBM immune …
substantial protumorigenic immune component. Knowledge of the GBM immune …