Optimization of long-term human iPSC-derived spinal motor neuron culture using a dendritic polyglycerol amine-based substrate

L Thiry, JP Clément, R Haag, TE Kennedy… - ASN neuro, 2022 - journals.sagepub.com
Human induced pluripotent stem cells (hiPSCs) derived from healthy and diseased
individuals can give rise to many cell types, facilitating the study of mechanisms of …

A simple method for poly-D-lysine coating to enhance adhesion and maturation of primary cortical neuron cultures in vitro

A Stil, B Liberelle, D Guadarrama Bello… - Frontiers in Cellular …, 2023 - frontiersin.org
Introduction Glass coverslips are used as a substrate since Harrison's initial nerve cell
culture experiments in 1910. In 1974, the first study of brain cells seeded onto polylysine …

Cell adhesion molecule immobilized gold surfaces for enhanced neuron‐electrode interfaces

B Aktas, A Ozgun, BD Kilickap… - Journal of Biomedical …, 2024 - Wiley Online Library
To provide a long‐term solution for increasing the biocompatibility of neuroprosthetics,
approaches to reduce the side effects of invasive neuro‐implantable devices are still in need …

A Bioinspired Astrocyte-Derived Coating Promotes the In Vitro Proliferation of Human Neural Stem Cells While Maintaining Their Stemness

AC Jimenez-Vergara, J Avina, TJ Block, A Sheldrake… - Biomimetics, 2023 - mdpi.com
The repair of neuronal tissue is a challenging process due to the limited proliferative
capacity of neurons. Neural stem cells (NSCs) can aid in the regeneration process of neural …

[HTML][HTML] High-throughput microcontact printing of proteins in microwell cell culture plates

D Chitsaz, TE Kennedy - MethodsX, 2024 - Elsevier
Microcontact printing (MCP) is used to pattern a surface with a specific compound, allowing
the spatially restricted response of cells to be assayed as they encounter a molecule of …

Phenotypic assay development with iPSC-derived neurons: technical considerations from plating to analysis

ED Buttermore - Phenotyping of Human iPSC-derived Neurons, 2023 - Elsevier
Induced pluripotent stem cell (iPSC)-derived neurons have been identified as a promising
model system to improve translation of preclinical neuroscience studies to the clinic. The use …