Biological applications of rare-earth based nanoparticles

C Bouzigues, T Gacoin, A Alexandrou - ACS nano, 2011 - ACS Publications
Biomedicine and cell and molecular biology require powerful imaging techniques of the
single molecule scale to the whole organism, either for fundamental science or diagnosis …

Lipid rafts: at a crossroad between cell biology and physics

K Jacobson, OG Mouritsen, RGW Anderson - Nature cell biology, 2007 - nature.com
Membrane lateral heterogeneity is accepted as a requirement for the function of biological
membranes and the notion of lipid rafts gives specificity to this broad concept. However, the …

Probing gene expression in live cells, one protein molecule at a time

J Yu, J Xiao, X Ren, K Lao, XS Xie - Science, 2006 - science.org
We directly observed real-time production of single protein molecules in individual
Escherichia coli cells. A fusion protein of a fast-maturing yellow fluorescent protein (YFP) …

Advances in single-molecule fluorescence methods for molecular biology

C Joo, H Balci, Y Ishitsuka, C Buranachai… - Annu. Rev …, 2008 - annualreviews.org
Ever since their introduction two decades ago, single-molecule (SM) fluorescence methods
have matured and branched out to address numerous biological questions, which were …

Droplet interface bilayers

H Bayley, B Cronin, A Heron, MA Holden… - Molecular …, 2008 - pubs.rsc.org
Droplet interface bilayers - Molecular BioSystems (RSC Publishing) DOI:10.1039/B808893D
Royal Society of Chemistry View PDF VersionPrevious ArticleNext Article DOI: 10.1039/B808893D …

[PDF][PDF] Single-molecule microscopy reveals plasma membrane microdomains created by protein-protein networks that exclude or trap signaling molecules in T cells

AD Douglass, RD Vale - Cell, 2005 - cell.com
Membrane subdomains have been implicated in T cell signaling, although their properties
and mechanisms of formation remain controversial. Here, we have used single-molecule …

Single-molecule fluorescence studies of protein folding and conformational dynamics

X Michalet, S Weiss, M Jäger - Chemical reviews, 2006 - ACS Publications
Single-molecule measurements provide unique information on heterogeneous populations
of molecules: They give access to the complete distribution of observables (rather than only …

Quantitative colocalization analysis of multicolor confocal immunofluorescence microscopy images: pushing pixels to explore biological phenomena

V Zinchuk, O Zinchuk, T Okada - Acta histochemica et cytochemica, 2007 - jstage.jst.go.jp
抄録 Quantitative colocalization analysis is an advanced digital imaging tool to examine
antigens of interest in immunofluorescence images obtained using confocal microscopes. It …

The spread of Ras activity triggered by activation of a single dendritic spine

CD Harvey, R Yasuda, H Zhong, K Svoboda - Science, 2008 - science.org
In neurons, individual dendritic spines isolate N-methyl-d-aspartate (NMDA) receptor–
mediated calcium ion (Ca2+) accumulations from the dendrite and other spines. However …

Sub-resolution lipid domains exist in the plasma membrane and regulate protein diffusion and distribution

DM Owen, DJ Williamson, A Magenau… - Nature communications, 2012 - nature.com
Lipid microdomains are postulated to regulate many membrane-associated processes but
have remained highly controversial. Here we provide the first direct evidence that the …