Replication-deficient viruses have been used most successfully in the field of gene therapy
because of their high transfection efficiency. However, the risk of insertional mutagenesis …

Over a decade ago, a landmark study that reported derivation of induced Pluripotent Stem
Cells (iPSCs) by reprogramming fibroblasts has transformed stem cell research attracting …

Induced pluripotent stem cells (iPSC) have been generated from somatic cells by
introducing reprogramming factors. Integration of foreign genes into the host genome is a …

Direct cardiac reprogramming holds great promise for regenerative medicine. We previously
generated directly reprogrammed induced cardiomyocyte-like cells (iCMs) by …

We have recently shown that non-viral gene therapy can stabilise the decline of lung
function in patients with cystic fibrosis (CF). However, the effect was modest, and more …

The generation of induced pluripotent stem cells (iPSCs) from differentiated mature cells is
one of the most promising technologies in the field of regenerative medicine. The ability to …

The cystic fibrosis transmembrane conductance regulator (CFTR) gene was identified in
1989. This opened the door for the development of cystic fibrosis (CF) gene therapy, which …

Gene therapy for cystic fibrosis (CF) is making encouraging progress into clinical trials.
However, further improvements in transduction efficiency are desired. To develop a novel …

Postnatal and adult human and monkey fibroblasts were infected with Sendai virus
containing the Yamanaka factors for 24 hr, then they were cultured in a chemically defined …

Gene therapy is currently being developed for a wide range of acute and chronic lung
diseases. The target cells, and to a degree the extra and intra-cellular barriers, are disease …