Why U matters: detection and functions of pseudouridine modifications in mRNAs
R Rodell, N Robalin, NM Martinez - Trends in Biochemical Sciences, 2024 - cell.com
The uridine modifications pseudouridine (Ψ), dihydrouridine, and 5-methyluridine are
present in eukaryotic mRNAs. Many uridine-modifying enzymes are associated with human …
present in eukaryotic mRNAs. Many uridine-modifying enzymes are associated with human …
Prediction of m6A and m5C at single-molecule resolution reveals a transcriptome-wide co-occurrence of RNA modifications
P Acera Mateos, AJ Sethi, A Ravindran… - Nature …, 2024 - nature.com
The epitranscriptome embodies many new and largely unexplored functions of RNA. A
significant roadblock hindering progress in epitranscriptomics is the identification of more …
significant roadblock hindering progress in epitranscriptomics is the identification of more …
De novo basecalling of m6A modifications at single molecule and single nucleotide resolution
ABSTRACT RNA modifications hold pivotal roles in shaping the fate and function of RNA
molecules. Although nanopore sequencing technologies have proven successful at …
molecules. Although nanopore sequencing technologies have proven successful at …
Nanopore signal deviations from pseudouridine modifications in RNA are sequence-specific: quantification requires dedicated synthetic controls
Chemical modifications to mRNA respond dynamically to environmental cues and are
important modulators of gene expression. Nanopore direct RNA sequencing has been …
important modulators of gene expression. Nanopore direct RNA sequencing has been …
Simultaneous identification of m6A and m5C reveals coordinated RNA modification at single-molecule resolution
The epitranscriptome embodies many new and largely unexplored functions of RNA. A
major roadblock in the epitranscriptomics field is the lack of transcriptome-wide methods to …
major roadblock in the epitranscriptomics field is the lack of transcriptome-wide methods to …
[PDF][PDF] Prediction of m6A and m5C at single-molecule resolution reveals a co-occurrence of RNA modifications across the transcriptome
PA Mateos, AJ Sethi, A Ravindran, A Srivastava… - Nat Commun, 2024 - d-nb.info
The epitranscriptome embodies many new and largely unexplored functions of RNA. A
significant roadblock hindering progress in epitranscriptomics is the identification of more …
significant roadblock hindering progress in epitranscriptomics is the identification of more …
Probing enzyme-dependent pseudouridylation using direct RNA sequencing to assess neuronal epitranscriptome plasticity
Chemical modifications in mRNAs, such as pseudouridine (psi), can control gene
expression. Yet, we know little about how they are regulated, especially in neurons. We …
expression. Yet, we know little about how they are regulated, especially in neurons. We …
A comprehensive survey of RNA modifications in a human transcriptome
L Mulroney, L Coscujuela-Tarrero, P Maragno… - bioRxiv, 2024 - biorxiv.org
RNA modifications are critical for transcript function and regulation. Direct RNA nanopore
sequencing offers a unique advantage by observing these modifications through …
sequencing offers a unique advantage by observing these modifications through …
Common analysis of direct RNA sequencinG CUrrently leads to misidentification of m5C at GCU motifs
KJ Watson, RE Bromley, BC Sparklin… - Life science …, 2024 - life-science-alliance.org
RNA modifications, such as methylation, can be detected with Oxford Nanopore
Technologies direct RNA sequencing. One commonly used tool for detecting 5 …
Technologies direct RNA sequencing. One commonly used tool for detecting 5 …
mRNA psi profiling using nanopore DRS reveals cell type-specific pseudouridylation
Pseudouridine (psi) is one of the most abundant human mRNA modifications generated
from the isomerization of uridine via psi synthases, including TRUB1 and PUS7. Nanopore …
from the isomerization of uridine via psi synthases, including TRUB1 and PUS7. Nanopore …