The spectroscopy of nitrogenases

C Van Stappen, L Decamps, GE Cutsail III… - Chemical …, 2020 - ACS Publications
Nitrogenases are responsible for biological nitrogen fixation, a crucial step in the
biogeochemical nitrogen cycle. These enzymes utilize a two-component protein system and …

New developments in high field electron paramagnetic resonance with applications in structural biology

M Bennati, TF Prisner - Reports on Progress in Physics, 2005 - iopscience.iop.org
Recent developments in microwave technologies have led to a renaissance of electron
paramagnetic resonance (EPR) due to the implementation of new spectrometers operating …

19F Electron-Nuclear Double Resonance Reveals Interaction between Redox-Active Tyrosines across the α/β Interface of E. coli Ribonucleotide Reductase

A Meyer, A Kehl, C Cui, FAK Reichardt… - Journal of the …, 2022 - ACS Publications
Ribonucleotide reductases (RNRs) catalyze the reduction of ribonucleotides to
deoxyribonucleotides, thereby playing a key role in DNA replication and repair. Escherichia …

Two closed ATP-and ADP-dependent conformations in yeast Hsp90 chaperone detected by Mn (II) EPR spectroscopic techniques

A Giannoulis, A Feintuch, Y Barak… - Proceedings of the …, 2020 - National Acad Sciences
Hsp90 plays a central role in cell homeostasis by assisting folding and maturation of a large
variety of clients. It is a homo-dimer, which functions via hydrolysis of ATP-coupled to …

Measurement of Angstrom to Nanometer Molecular Distances with 19F Nuclear Spins by EPR/ENDOR Spectroscopy

A Meyer, S Dechert, S Dey… - Angewandte Chemie …, 2020 - Wiley Online Library
Spectroscopic and biophysical methods for structural determination at atomic resolution are
fundamental in studies of biological function. Here we introduce an approach to measure …

57Fe ENDOR Spectroscopy and 'Electron Inventory' Analysis of the Nitrogenase E4 Intermediate Suggest the Metal-Ion Core of FeMo-Cofactor Cycles Through Only …

PE Doan, J Telser, BM Barney… - Journal of the …, 2011 - ACS Publications
N2 binds to the active-site metal cluster in the nitrogenase MoFe protein, the FeMo-cofactor
([7Fe-9S-Mo-homocitrate-X]; FeMo-co) only after the MoFe protein has accumulated three or …

Hydrogen Bond Network between Amino Acid Radical Intermediates on the Proton-Coupled Electron Transfer Pathway of E. coli α2 Ribonucleotide Reductase

TU Nick, W Lee, S Koßmann, F Neese… - Journal of the …, 2015 - ACS Publications
Ribonucleotide reductases (RNRs) catalyze the conversion of ribonucleotides to
deoxyribonucleotides in all organisms. In all Class Ia RNRs, initiation of nucleotide …

New synthesis and insight into the structure of blue ultramarine pigments

D Arieli, DEW Vaughan, D Goldfarb - Journal of the American …, 2004 - ACS Publications
A new and easy method for preparing blue sodalite pigments which involves high-
temperature calcination of sodalite samples synthesized with aluminum sulfate and an …

Increasing sensitivity of pulse EPR experiments using echo train detection schemes

F Mentink-Vigier, A Collauto, A Feintuch… - Journal of Magnetic …, 2013 - Elsevier
Modern pulse EPR experiments are routinely used to study the structural features of
paramagnetic centers. They are usually performed at low temperatures, where relaxation …

Spectrometer manager: A versatile control software for pulse EPR spectrometers

B Epel, I Gromov, S Stoll, A Schweiger… - Concepts in Magnetic …, 2005 - Wiley Online Library
A versatile control software for pulse EPR spectrometers is introduced. Common and task‐
specific problems are discussed and their solutions are described. The software provides …