An introduction to the analysis of shotgun metagenomic data

TJ Sharpton - Frontiers in plant science, 2014 - frontiersin.org
Environmental DNA sequencing has revealed the expansive biodiversity of microorganisms
and clarified the relationship between host-associated microbial communities and host …

Experimental and analytical tools for studying the human microbiome

J Kuczynski, CL Lauber, WA Walters… - Nature Reviews …, 2012 - nature.com
The human microbiome substantially affects many aspects of human physiology, including
metabolism, drug interactions and numerous diseases. This realization, coupled with ever …

phyloseq: an R package for reproducible interactive analysis and graphics of microbiome census data

PJ McMurdie, S Holmes - PloS one, 2013 - journals.plos.org
Background The analysis of microbial communities through DNA sequencing brings many
challenges: the integration of different types of data with methods from ecology, genetics …

Structure, function and diversity of the healthy human microbiome

nature, 2012 - nature.com
Studies of the human microbiome have revealed that even healthy individuals differ
remarkably in the microbes that occupy habitats such as the gut, skin and vagina. Much of …

Strengths and limitations of 16S rRNA gene amplicon sequencing in revealing temporal microbial community dynamics

R Poretsky, LM Rodriguez-R, C Luo, D Tsementzi… - PloS one, 2014 - journals.plos.org
This study explored the short-term planktonic microbial community structure and resilience
in Lake Lanier (GA, USA) while simultaneously evaluating the technical aspects of …

Biodiversity soup: metabarcoding of arthropods for rapid biodiversity assessment and biomonitoring

DW Yu, Y Ji, BC Emerson, X Wang, C Ye… - Methods in Ecology …, 2012 - Wiley Online Library
Traditional biodiversity assessment is costly in time, money and taxonomic expertise.
Moreover, data are frequently collected in ways (eg visual bird lists) that are unsuitable for …

[HTML][HTML] PhyloSift: phylogenetic analysis of genomes and metagenomes

AE Darling, G Jospin, E Lowe, FA Matsen IV, HM Bik… - PeerJ, 2014 - peerj.com
Like all organisms on the planet, environmental microbes are subject to the forces of
molecular evolution. Metagenomic sequencing provides a means to access the DNA …

A perspective on 16S rRNA operational taxonomic unit clustering using sequence similarity

NP Nguyen, T Warnow, M Pop, B White - NPJ biofilms and microbiomes, 2016 - nature.com
The standard pipeline for 16S amplicon analysis starts by clustering sequences within a
percent sequence similarity threshold (typically 97%) into 'Operational Taxonomic …

Toward accurate and quantitative comparative metagenomics

S Nayfach, KS Pollard - Cell, 2016 - cell.com
Shotgun metagenomics and computational analysis are used to compare the taxonomic and
functional profiles of microbial communities. Leveraging this approach to understand roles of …

Role of the intestinal microbiome in health and disease: from correlation to causation

WM de Vos, EAJ de Vos - Nutrition reviews, 2012 - academic.oup.com
Recorded observations indicating an association between intestinal microbes and health
are long-standing in terms of specific diseases, but emerging high-throughput technologies …