An introduction to the analysis of shotgun metagenomic data
TJ Sharpton - Frontiers in plant science, 2014 - frontiersin.org
Environmental DNA sequencing has revealed the expansive biodiversity of microorganisms
and clarified the relationship between host-associated microbial communities and host …
and clarified the relationship between host-associated microbial communities and host …
Experimental and analytical tools for studying the human microbiome
The human microbiome substantially affects many aspects of human physiology, including
metabolism, drug interactions and numerous diseases. This realization, coupled with ever …
metabolism, drug interactions and numerous diseases. This realization, coupled with ever …
phyloseq: an R package for reproducible interactive analysis and graphics of microbiome census data
PJ McMurdie, S Holmes - PloS one, 2013 - journals.plos.org
Background The analysis of microbial communities through DNA sequencing brings many
challenges: the integration of different types of data with methods from ecology, genetics …
challenges: the integration of different types of data with methods from ecology, genetics …
Structure, function and diversity of the healthy human microbiome
nature, 2012 - nature.com
Studies of the human microbiome have revealed that even healthy individuals differ
remarkably in the microbes that occupy habitats such as the gut, skin and vagina. Much of …
remarkably in the microbes that occupy habitats such as the gut, skin and vagina. Much of …
Strengths and limitations of 16S rRNA gene amplicon sequencing in revealing temporal microbial community dynamics
This study explored the short-term planktonic microbial community structure and resilience
in Lake Lanier (GA, USA) while simultaneously evaluating the technical aspects of …
in Lake Lanier (GA, USA) while simultaneously evaluating the technical aspects of …
Biodiversity soup: metabarcoding of arthropods for rapid biodiversity assessment and biomonitoring
Traditional biodiversity assessment is costly in time, money and taxonomic expertise.
Moreover, data are frequently collected in ways (eg visual bird lists) that are unsuitable for …
Moreover, data are frequently collected in ways (eg visual bird lists) that are unsuitable for …
[HTML][HTML] PhyloSift: phylogenetic analysis of genomes and metagenomes
Like all organisms on the planet, environmental microbes are subject to the forces of
molecular evolution. Metagenomic sequencing provides a means to access the DNA …
molecular evolution. Metagenomic sequencing provides a means to access the DNA …
A perspective on 16S rRNA operational taxonomic unit clustering using sequence similarity
The standard pipeline for 16S amplicon analysis starts by clustering sequences within a
percent sequence similarity threshold (typically 97%) into 'Operational Taxonomic …
percent sequence similarity threshold (typically 97%) into 'Operational Taxonomic …
Toward accurate and quantitative comparative metagenomics
S Nayfach, KS Pollard - Cell, 2016 - cell.com
Shotgun metagenomics and computational analysis are used to compare the taxonomic and
functional profiles of microbial communities. Leveraging this approach to understand roles of …
functional profiles of microbial communities. Leveraging this approach to understand roles of …
Role of the intestinal microbiome in health and disease: from correlation to causation
WM de Vos, EAJ de Vos - Nutrition reviews, 2012 - academic.oup.com
Recorded observations indicating an association between intestinal microbes and health
are long-standing in terms of specific diseases, but emerging high-throughput technologies …
are long-standing in terms of specific diseases, but emerging high-throughput technologies …